Streptococcus pneumoniae augments inflammatory process in asthma model without changing Th2 profile

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dc.contributorCentro de Biotecnologiapt_BR
dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorLaia, Roseane M.pt_BR
dc.contributor.authorCastro, Thamyres B. P.pt_BR
dc.contributor.authorSantos, Camila A.pt_BR
dc.contributor.authorSaraiva-Romanholo, Beatriz M.pt_BR
dc.contributor.authorOliveira, Maria Leonor Sarno dept_BR
dc.contributor.authorMiyaji, Eliane Namiept_BR
dc.contributor.authorTiberio, Iolanda F.C.L.pt_BR
dc.contributor.authorOlivo, Clarice Rosapt_BR
dc.identifier.citationLaia RM., Castro TB.P., Santos CA., Saraiva-Romanholo BM., Oliveira MLS, Miyaji EN, et al. Streptococcus pneumoniae augments inflammatory process in asthma model without changing Th2 profile. Eur Respir J. 2020; 56(64):3318. doi:10.1183/13993003.congress-2020.3318.pt_BR
dc.description.abstractStudies suggested that some aspects of asthma exacerbation by Spn infection remain unclear. Objective: to evaluate possible mechanism that worsen inflammation caused by Spn in an experimental model of chronic allergic inflammation. Methods: 30 BALB/c mice were divided in 4 groups: SAL (non-sensitized group), STREP (animals challenged with Spn), OVA (ovalbumin sensitized group), OVAST (OVA sensitized and challenged with Spn). OVA and OVAST groups received intraperitoneal injections of ovalbumin (OVA) solution (days 1 and 14). OVA challenges were performed on days 22, 24, 26, and 28. Afterwards, animals were challenged with pneumococcal strains M10 (11A)(50ul/bacteria in saline). After 12h, lung mechanics and bronchoalveolar lavage (BAL) were performed. Animals were euthanized, lungs removed for immunohistochemistry and morphometric analysis. Results: Challenge with Spn in OVA sensitized group induces an increase in of total cells (46.33±13.22x104cells/mL), neutrophils (23.70±14.39x104cells/mL), macrophages (7.70±2.03x104cells/mL) and eosinophils (14.52±13.88x104cells/mL) in BALF as well as increasing in polymorphonuclear cells (0.152±0.06mm2) and expression of IL-17 (12.12±2.67mm2) in peribronchovascular area in lung compared to OVA group (p<0.05). There were an increase in tissue damping (27.01±7.25cmH2O/mL/s(1-a)); expression of IL-5 (10.15±3.39mm2) and IL-13 (8.85±3.56mm2) in peribronchovascular area were observed in OVA groups compared to other groups (p<0.05). Conclusion: Challenge with Spn, in this model induces an increasing in lung inflammation by increasing IL-17 without changes in Th2 profile.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipUniversidade de São Paulo (USP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.relation.ispartofThe European Respiratory Journalpt_BR
dc.titleStreptococcus pneumoniae augments inflammatory process in asthma model without changing Th2 profilept_BR
dc.contributor.externalInstituto de Assistência Médica ao Servidor Público Estadual de S. Paulo (IAMSPE)pt_BR
dc.contributor.externalUniversidade Cidade de São Paulo (UNICID)pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.relation.ispartofabbreviatedEur Respir Jpt_BR
dc.identifier.citationabntv, 56, n. 64, 3318, 2020pt_BR
dc.identifier.citationvancouver2020; 56(64):3318pt_BR
dc.contributor.butantanOliveira, Maria Leonor Sarno de|:Pesquisador|:Centro de Biotecnologia|:Lab. Bacteriologiapt_BR
dc.contributor.butantanMiyaji, Eliane Namie|:Pesquisador|:Centro de Biotecnologia|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦19966-6pt_BR
dc.sponsorship.butantanUniversidade de São Paulo (USP)¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.description.internalResumo de congresso.pt_BR
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