Short-term cold storage of seminal samples of the golden lancehead pitviper (Bothrops insularis)

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dc.contributorLEEV - Laboratório de Ecologia e Evoluçãopt_BR
dc.contributor.authorSilva, Kalena Barros dapt_BR
dc.contributor.authorFrediani, Mayra Hespanholpt_BR
dc.contributor.authorAngrimani, Daniel de Souza Ramospt_BR
dc.contributor.authorCoeti, Rafaela Zanipt_BR
dc.contributor.authorPereira, Ricardo José Garciapt_BR
dc.contributor.authorNichi, Marcíliopt_BR
dc.contributor.authorAlmeida-Santos, Selma Mariapt_BR
dc.date.accessioned2021-02-16T17:47:54Z-
dc.date.available2021-02-16T17:47:54Z-
dc.date.issued2021pt_BR
dc.identifier.citationSilva KB, Frediani MH, Angrimani DSR, Coeti RZ, Pereira RJG, Nichi M, et al. Short-term cold storage of seminal samples of the golden lancehead pitviper (Bothrops insularis). Zoo Biol.. 2021 Feb;40(3):256-260. doi:10.1002/zoo.21590.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3537-
dc.description.abstractAssisted reproductive technologies such as cooling, freezing, and artificial insemination are essential in conserving genetic diversity of critically endangered reptiles like the golden lancehead pitviper (Bothrops insularis). Therefore, we examined viability of semen samples from captive golden lanceheads diluted in coconut‐water based extender over a 48‐h period of cooling (5°C). Semen evaluations were performed immediately after the dilution and at 6, 24, and 48 h, using computer‐assisted sperm analysis and stains to assess plasma membrane and acrosomal status. Our findings showed that the extender and protocol employed here were effective in preserving golden lancehead pitviper spermatozoa for a short periods of time (48 h), allowing semen samples to be transported to distant locations for immediate use without the setbacks of cryopreservation.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent256-260pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofZoo Biologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleShort-term cold storage of seminal samples of the golden lancehead pitviper (Bothrops insularis)pt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1002/zoo.21590pt_BR
dc.identifier.urlhttps://doi.org/10.1002/zoo.21590pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume40pt_BR
dc.identifier.citationissue3pt_BR
dc.subject.keywordextenderspt_BR
dc.subject.keywordreproductionpt_BR
dc.subject.keywordsnakespt_BR
dc.subject.keywordspermatozoapt_BR
dc.subject.keywordsquamatapt_BR
dc.relation.ispartofabbreviatedZoo Biol.pt_BR
dc.identifier.citationabntv. 40, n. 3, p. 256-260, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Feb;40(3):256-260pt_BR
dc.contributor.butantanSilva, Kalena Barros da|:Aluno|:LEEV - Laboratório de Ecologia e Evolução|:PrimeiroAutorpt_BR
dc.contributor.butantanCoeti, Rafaela Zani|:Aluno|:LEEV - Laboratório de Ecologia e Evolução|:Autor de correspondênciapt_BR
dc.contributor.butantanAlmeida-Santos, Selma Maria|:Pesquisador|:LEEV - Laboratório de Ecologia e Evoluçãopt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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