Small intestine remodeling in male Goto-Kakizaki rats

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dc.contributor.authorPereira, Joice Naiara Bertagliapt_BR
dc.contributor.authorMurata, Gilson Masahiropt_BR
dc.contributor.authorSato, Fabio Takeopt_BR
dc.contributor.authorMarosti, Aline Rosapt_BR
dc.contributor.authorCarvalho, Carla Roberta de Oliveirapt_BR
dc.contributor.authorCuri, Ruipt_BR
dc.date.accessioned2021-02-16T18:19:44Z-
dc.date.available2021-02-16T18:19:44Z-
dc.date.issued2021pt_BR
dc.identifier.citationPereira JNB, Murata GM, Sato FT, Marosti AR, Carvalho CRO, Curi R. Small intestine remodeling in male Goto-Kakizaki rats. Physiol. Rep.. 2021 Fev;9(3):. doi:10.14814phy2.14755.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3538-
dc.description.abstractBackground: Obesity is associated with the development of insulin resistance (IR) and type‐2 diabetes mellitus (T2DM); however, not all patients with T2DM are obese. The Goto–Kakizaki (GK) rat is an experimental model of spontaneous and non‐obese T2DM. There is evidence that the intestine contributes to IR development in GK animals. This information prompted us to investigate small intestine remodeling in this animal model. Methods: Four‐month‐old male Wistar (control) and GK rats were utilized for the present study. After removing the small intestine, the duodenum, proximal jejunum, and distal ileum were separated. We then measured villi and muscular and mucosa layer histomorphometry, goblet cells abundance, total myenteric and submucosal neuron populations, and inflammatory marker expression in the small intestinal segments and intestinal transit of both groups of animals. Key Results: We found that the GK rats exhibited decreased intestinal area (p < 0.0001), decreased crypt depth in the duodenum (p = 0.01) and ileum (p < 0.0001), increased crypt depth in the jejunum (p < 0.0001), longer villi in the jejunum and ileum (p < 0.0001), thicker villi in the duodenum (p < 0.01) and ileum (p < 0.0001), thicker muscular layers in the duodenum, jejunum, and ileum (p < 0.0001), increased IL‐1β concentrations in the duodenum and jejunum (p < 0.05), and increased concentrations of NF‐κB p65 in the duodenum (p < 0.01), jejunum and ileum (p < 0.05). We observed high IL‐1β reactivity in the muscle layer, myenteric neurons, and glial cells of the experimental group. GK rats also exhibited a significant reduction in submucosal neuron density in the jejunum and ileum, ganglionic hypertrophy in all intestinal segments studied (p < 0.0001), and a slower intestinal transit (about 25%) compared to controls. Conclusions: The development of IR and T2DM in GK rats is associated with small intestine remodeling that includes marked alterations in small intestine morphology, local inflammation, and reduced intestinal transit.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extente14755pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPhysiological Reportspt_BR
dc.rightsOpen Accesspt_BR
dc.titleSmall intestine remodeling in male Goto-Kakizaki ratspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.14814phy2.14755pt_BR
dc.identifier.urlhttps://doi.org/10.14814phy2.14755pt_BR
dc.contributor.externalUniversidade Cruzeiro do Sul (UNICSUL)pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversidade Estadual de Campinas (UNICAMP)pt_BR
dc.contributor.externalUniversidade Estadual de Maringá (UEM)pt_BR
dc.identifier.citationvolume9pt_BR
dc.identifier.citationissue3pt_BR
dc.subject.keywordenteric nervous systempt_BR
dc.subject.keywordGK ratspt_BR
dc.subject.keywordinflammationpt_BR
dc.subject.keywordsmall intestine morphologypt_BR
dc.subject.keywordtype 2 diabetespt_BR
dc.relation.ispartofabbreviatedPhysiol Reppt_BR
dc.identifier.citationabntv.9, n. 3, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Fev;9(3):pt_BR
dc.contributor.butantanCuri, Rui|:Diretorpt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/09868‐7pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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item.languageiso639-1English-
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