Patagonin-CRISP: antimicrobial activity and source of antimicrobial molecules in duvernoy's gland secretion (Philodryas patagoniensis Snake):

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dc.contributorLab. Parasitologiapt_BR
dc.contributor(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributor(CeTICS) Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributorLab. Herpetologiapt_BR
dc.contributor.authorBadari, Juliana Cuocopt_BR
dc.contributor.authorDíaz-Roa, Andreapt_BR
dc.contributor.authorRocha, Marisa Maria Teixeira dapt_BR
dc.contributor.authorMendonça, Ronaldo Zucatellipt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.date.accessioned2021-02-23T17:30:39Z-
dc.date.available2021-02-23T17:30:39Z-
dc.date.issued2021pt_BR
dc.identifier.citationBadari JC, D-RA, Rocha MMT, Mendonça RZ, Silva Junior PI. Patagonin-CRISP: Antimicrobial Activity and source of antimicrobial molecules in Duvernoy's gland secretion (Philodryas patagoniensis Snake). Front. Pharmacol.. 2021 Fev;11:586705. doi:10.3389/fphar.2020.586705.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3564-
dc.description.abstractSnake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known about their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of Duvernoy's gland of the rear-fanged Philodryas patagoniensis. Fractions of this venom were subjected to reverse-phase high performance liquid chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS/MS, and a protein of 24,848.8 Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of P. patagoniensis had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for P. patagoniensis venom or for a CRISP family protein.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent586705pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Pharmacologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titlePatagonin-CRISP: antimicrobial activity and source of antimicrobial molecules in duvernoy's gland secretion (Philodryas patagoniensis Snake):pt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fphar.2020.586705pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fphar.2020.586705pt_BR
dc.contributor.external(UNAD) Universidad Nacional Abierta y a Distanciapt_BR
dc.identifier.citationvolume11pt_BR
dc.subject.keywordCRISPpt_BR
dc.subject.keywordantimicrobialpt_BR
dc.subject.keywordbioprospectingpt_BR
dc.subject.keyworddipsadidaept_BR
dc.subject.keywordsnakespt_BR
dc.subject.keywordtoxinpt_BR
dc.subject.keywordpatagonin-CRISPpt_BR
dc.relation.ispartofabbreviatedFront Pharmacolpt_BR
dc.identifier.citationabntv. 11, 586705, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Fev;11:586705pt_BR
dc.contributor.butantanBadari, Juliana Cuoco|:Técnico|:Lab. Parasitologia|:PrimeiroAutorpt_BR
dc.contributor.butantanDíaz-Roa, Andrea|:Aluno|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributor.butantanMendonça, Ronaldo Zucatelli|:Pesquisador:Docente Permanente PPGTOX|:Lab. Parasitologiapt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):(LETA) Lab. Toxinologia Aplicada|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/24867-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦ 2013/07467-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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