Variações proteômicas do efeito da peçonha de Bothrops jararaca em linhagens celulares tumorais de mama.

Abstract

Cancer is characterized by unnatural cell growth that can result from genetic mutations escaping the apoptosis process. Cancer cells invade adjacent or distant tissues and, added to the process of angiogenesis and metastasis, they clump together to form the tumor. There are currently some papers describing the antitumorigenic biochemical characteristics of snake venom that claim that this type of venom is capable of inhibiting cell proliferation and promoting cell death by different means. However, there is no work characterizing the proteomic molecular effect of the tumor cell lines treatment with snake venom. In this work, we characterized the comparative, functional and quantitative differential proteome of the MCF-7 and MDA-MB231 tumor cell lines treated with Bothrops jararaca venom at different concentrations. Based on data obtained from the cytotoxic assays of B. jararaca venom treatment in these cells we subjected these lineages to low (0.63 μg / mL) and high / subcitotoxicity (2.5 μg / mL) doses of venom for 24 h. The cells were subjected to ice-cold 8M urea cell lysis, reduction and alkylation, trypsin digestion and desalination for analysis by high resolution liquid chromatography coupled to mass spectrometry (nLC-MS / MS). In order to identify, quantify, characterize and compare the functional and biochemical proteins profile in which abundance changed significantly after venom treatment, we used MaxQuant, Perseus and PantherDB, Reactome and String software for data analysis. These analyzes show the differential expression of various proteins, such as histone H3, SNX3, HEL-S-156an, MCC2 and GSTM3. Several of these proteins play important cancer-related roles, such as cell proliferation, invasion, metastasis, apoptosis and stress response. Therefore, these data show that the venom or some of its components have a potential use for cancer therapy and may induce a homeostatic imbalance in cancer cells.