GroEL protein of the Leptospira spp. interacts with host proteins and induces cytokines secretion on macrophages

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dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorHo, Joana Diaspt_BR
dc.contributor.authorTakara, Luiz Eduardo Massaopt_BR
dc.contributor.authorMonaris, Denizept_BR
dc.contributor.authorGonçalves, Aline Patríciapt_BR
dc.contributor.authorSouza-Filho, Antonio Franciscopt_BR
dc.contributor.authorSouza, Gisele Oliveira dept_BR
dc.contributor.authorHeinemann, Marcos Bryanpt_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorAbreu, Patricia Antonia Estimapt_BR
dc.identifier.citationHo JD, Takara LEM, Monaris D, Gonçalves AP, Souza-Filho AF, Souza GO, et al. GroEL protein of the Leptospira spp. interacts with host proteins and induces cytokines secretion on macrophages. BMC Microbiol.. 2021 Mar;21:99. doi:10.1186/s12866-021-02162-w.pt_BR
dc.description.abstractBackground Leptospirosis is a zoonotic disease caused by infection with spirochetes from Leptospira genus. It has been classified into at least 17 pathogenic species, with more than 250 serologic variants. This wide distribution may be a result of leptospiral ability to colonize the renal tubules of mammalian hosts, including humans, wildlife, and many domesticated animals. Previous studies showed that the expression of proteins belonging to the microbial heat shock protein (HSP) family is upregulated during infection and also during various stress stimuli. Several proteins of this family are known to have important roles in the infectious processes in other bacteria, but the role of HSPs in Leptospira spp. is poorly understood. In this study, we have evaluated the capacity of the protein GroEL, a member of HSP family, of interacting with host proteins and of stimulating the production of cytokines by macrophages. Results The binding experiments demonstrated that the recombinant GroEL protein showed interaction with several host components in a dose-dependent manner. It was also observed that GroEL is a surface protein, and it is secreted extracellularly. Moreover, two cytokines (tumor necrosis factor-α and interleukin-6) were produced when macrophages cells were stimulated with this protein. Conclusions Our findings showed that GroEL protein may contribute to the adhesion of leptospires to host tissues and stimulate the production of proinflammatory cytokines during infection. These features might indicate an important role of GroEL in the pathogen-host interaction in the leptospirosis.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.relation.ispartofBMC Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleGroEL protein of the Leptospira spp. interacts with host proteins and induces cytokines secretion on macrophagespt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.subject.keywordChaperonin 60pt_BR
dc.subject.keywordJ774.1 cellspt_BR
dc.subject.keywordMoonlighting proteinpt_BR
dc.relation.ispartofabbreviatedBMC Microbiol.pt_BR
dc.identifier.citationabntv. 21, 99, mar. 2021pt_BR
dc.identifier.citationvancouver2021 Mar;21:99pt_BR
dc.contributor.butantanHo, Joana Dias|:Aluno|:Laboratório de Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanTakara, Luiz Eduardo Massao|:Aluno|:Laboratório de Bacteriologiapt_BR
dc.contributor.butantanMonaris, Denize|:Aluno|:Laboratório de Bacteriologiapt_BR
dc.contributor.butantanGonçalves, Aline Patrícia|:Aluno|:Laboratório de Bacteriologiapt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrialpt_BR
dc.contributor.butantanAbreu, Patricia Antonia Estima|:Pesquisador|:Laboratório de Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2010/51215–9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2019/00546–0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2019/09804–1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
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