Avaliação comparativa do plasma hiperimune nas três sangrias de produção dos cavalos soroprodutores da Fazenda São Joaquim

Abstract

Hyperimmune plasma is the raw material for the production of heterologous serum, which corresponds to the main specific treatment against toxins from microorganisms and venomous animals. This work aims to perform a comparative analysis of the plasma collected in the three production bleeds, subjectively analyzing the production of immunoglobulins through the amount of total protein and measuring the levels of pH, glucose, leukocytes, density and fibrinogen. Samples were collected from 67 horses, divided into three groups according to the antigen to which they were immunized, being: Group 1 (G1) - immunized against tetanus toxoid, Group 2 (G2) - immunized against crotalic antigen and Group 3 ( G3) - immunized against scorpionic antigen. Samples were obtained during production bleeding procedures, by collecting whole blood in vacuum tubes with EDTA. The analyzes were performed using a refractometer to quantify total protein, density and fibrinogen and reagent strips for measuring pH, leukocytes and glucose. The results demonstrate significant variations between the three services studied, mainly in relation to total protein, where in G1 its peak production occurred in the second bleed, in G2 it remained stable in the first two bleeds with a slight drop in the third, and in G3 with peak in the first production bleed. Through the rapid tests proposed in this study it is possible to have an overview of the divergence of parameters between the three production bleeds, quickly and at a low cost, but to obtain more reliable results, especially with regard to immunoglobulins, indicated the adoption of more specific analysis techniques.