Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation

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dc.contributorSeção de Vacinas Anaeróbicaspt_BR
dc.contributorLab. Biofísicapt_BR
dc.contributor.authorSartori, Giselle Pacificopt_BR
dc.contributor.authorCosta, Andréa dapt_BR
dc.contributor.authorSantos, Fernandapt_BR
dc.contributor.authorMariano, Douglas Oscar Ceolinpt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorSpencer, Patrick Jackpt_BR
dc.contributor.authorNali, Luiz Henrique da Silvapt_BR
dc.contributor.authorGalisteo Jr, Andrés Jimenezpt_BR
dc.date.accessioned2021-05-14T20:05:28Z-
dc.date.available2021-05-14T20:05:28Z-
dc.date.issued2021pt_BR
dc.identifier.citationSartori GP, Costa A, Santos F, Mariano DOC, Pimenta DC, Spencer PJ, et al. Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiation. J Venom Anim Toxins Incl Trop Dis. 2021 Apr;27:e20200140. doi:10.1590/1678-9199-jvatitd-2020-0140.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3734-
dc.description.abstractBackground: Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the post-vaccination side effects. Therefore, the aim of this study was to characterize the tetanus toxin structure after different doses of ionizing radiation of 60Co. Methods: Irradiated and native tetanus toxin was characterized by SDS PAGE in reducing and non-reducing conditions and MALD-TOF. Enzymatic activity was measured by FRET substrate. Also, antigenic properties were assessed by ELISA and Western Blot data. Results: Characterization analysis revealed gradual modification on the tetanus toxin structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the identification up to the dose of 4 kGy. At subsequent doses, peptide identification was minimal. The analysis of the enzymatic activity by fluorescence showed 35 % attenuation in the activity even at higher doses. In the antigenic evaluation, anti-tetanus toxin antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels. Conclusion: Ionizing radiation promoted structural changes in the tetanus toxin such as fragmentation and/or aggregation and attenuation of enzymatic activity as the dose increased, but antigenic recognition of the toxin remained at good levels indicating its possible use as an immunogen. However, studies of enzymatic activity of tetanus toxin irradiated with doses above 8 kGy should be further analyzed.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extente20200140pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleCharacterization and evaluation of the enzymatic activity of tetanus toxin submitted to cobalt-60 gamma radiationpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1590/1678-9199-jvatitd-2020-0140pt_BR
dc.identifier.urlhttps://doi.org/10.1590/1678-9199-jvatitd-2020-0140pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalInstituto de Pesquisas Energéticas e Nucleares (IPEN)pt_BR
dc.contributor.externalUniversidade Santo Amaro (UNISA)pt_BR
dc.identifier.citationvolume27pt_BR
dc.subject.keywordRadiationpt_BR
dc.subject.keywordFragmentationpt_BR
dc.subject.keywordEnzymatic activitypt_BR
dc.subject.keywordGamma rayspt_BR
dc.relation.ispartofabbreviatedJ Venom Anim Toxins Incl Trop Dispt_BR
dc.identifier.citationabntv. 27, e20200140, abr. 2021pt_BR
dc.identifier.citationvancouver2021 Apr;27:e20200140pt_BR
dc.contributor.butantanSantos, Fernanda Lúcio do|:Colaborador:|Seção de vacinas anaeróbicaspt_BR
dc.contributor.butantanMariano, Douglas Oscar Ceolin|:Aluno|:Lab. Biofísicapt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente permanente PPGTOX|:Lab. Biofísicapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/26782-8pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦01.09.0278.04pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦01.12.0450.03pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301974/2019-5pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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