Insights into antimicrobial peptides from Limacus flavus Mucus

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dc.contributor(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributor(CeTICS) Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.authorHayashida, Patricia Yumipt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.date.accessioned2021-06-11T18:59:49Z-
dc.date.available2021-06-11T18:59:49Z-
dc.date.issued2021pt_BR
dc.identifier.citationHayashida PY, Silva Junior PI. Insights into Antimicrobial Peptides from Limacus flavus Mucus. Curr. Microbiol.. 2021 June;78:2970–2979. doi:10.1007/s00284-021-02552-3.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3828-
dc.description.abstractAntimicrobial peptides have been a major research subject since the rise of antimicrobial resistance as a major public health problem. These molecules are considered a potential therapeutic source of antibiotics with broad-spectrum activity against microorganisms. Two antimicrobial peptides were isolated from the mucus of the Limacus flavus slug. The mucus was obtained by thermal shock, lyophilized and extracted with acetic acid. The supernatant was prefractionated in Sep-Pak and shortly thereafter fractionated by reverse-phase high-performance liquid chromatography. The manually obtained fractions were used in antimicrobial and cytotoxic assays and finally subjected to mass spectrometry (MS/MS). Characterization was performed by bioinformatics analysis with the tool Peaks®X + and by comparison with the NCBI and UniProt-SwissProt databases. Additionally, the physicochemical parameters of the samples were evaluated with online programs. Two fractions comtained antimicrobial peptides with the ability to inhibit Micrococcus luteus A270; both samples, LFMP-001 and LFMP-002, were hydrophilic molecules consisting of fewer than 20 residues. Comparison of the SDS-PAGE and Peaks®X + data showed that both had Mw < 3 kDa. In summary, this study presents data on the isolation and characterization of antimicrobial peptides from a slug and shows their potential against gram-positive bacteria.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(USP) Universidade de São Paulopt_BR
dc.format.extent2970–2979pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofCurrent Microbiologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleInsights into antimicrobial peptides from Limacus flavus Mucuspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1007/s00284-021-02552-3pt_BR
dc.identifier.urlhttps://doi.org/10.1007/s00284-021-02552-3pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume78pt_BR
dc.relation.ispartofabbreviatedCurr Microbiolpt_BR
dc.identifier.citationabntv. 78, p. 2970–2979, jun. 2021pt_BR
dc.identifier.citationvancouver2021 June;78:2970–2979pt_BR
dc.contributor.butantanHayashida, Patricia Yumi|:Aluno|:(LETA) Lab. Toxinologia Aplicada:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutorpt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:(LETA) Lab. Toxinologia Aplicada:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦472744/2012-7pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantanUniversidade de São Paulo (USP)¦¦0000000406355304pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextSem Texto completo-
item.languageiso639-1English-
item.openairetypeArticle-
item.grantfulltextnone-
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crisitem.author.orcid0000-0001-6619-6489-
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