From birth to adulthood: an analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages

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dc.contributorLab. Herpetologiapt_BR
dc.contributor.authorHatakeyama, Daniela Mikipt_BR
dc.contributor.authorTasima, Lidia Jorgept_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorFabri, Carolinept_BR
dc.contributor.authorStuginski, Daniel Rodriguespt_BR
dc.contributor.authorSant'Anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorTashima, Alexandre Keijipt_BR
dc.contributor.authorNishiduka, Erika Sayuript_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.date.accessioned2021-06-24T18:22:26Z-
dc.date.available2021-06-24T18:22:26Z-
dc.date.issued2021pt_BR
dc.identifier.citationHatakeyama DM, Tasima LJ, Galizio NC, Silva CS, Fabri C, Stuginski DR, et al. From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages. PloS One. 2021 June;16(6):e0253050. doi:10.1371/journal.pone.0253050.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3859-
dc.description.abstractThe Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents a serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles’ bites were mainly related to coagulation, while those caused by adults’ bites had a more prominent local damage. In this work, we analyzed the venoms of B. moojeni at different life stages to better understand the ontogeny shift in this species. Snakes were grouped by age and sex, and venom pools were formed accordingly. Compositional analyses by one-dimensional electrophoresis (1-DE), chromatography, and mass spectrometry revealed that ontogenetic changes might be mostly related to phospholipase A2 (PLA2) and metalloproteases. Regarding the venoms functional aspect, proteolytic, L-amino acid oxidase, PLA2, and coagulant in vitro activities were assayed, but only the first and the last ones showed age-related changes, with the venom of snakes up to 1 year-old displaying lower proteolytic and higher coagulant activities, while those from 2 years-old onward presented the opposite relation. The venoms of 3 years-old snakes were exceptions to the compositional and functional pattern of adults as both venoms presented profiles similar to neonates. Sex-related differences were observed in specific groups and were not age-related. In vivo experiments (median lethal dose and hemorrhagic activity) were statistically similar between neonates and adults, however we verified that the adult venom killed mice faster comparing to the neonates. All venoms were mostly recognized by the antibothropic serum and displayed similar profiles to 1-DE in western blotting. In conclusion, the Brazilian lancehead venom showed ontogenetic shift in its composition and activities. Furthermore, this change occurred in snakes from 1 to 2 years-old, and interestingly the venom pools from 3 years-old snakes had particular characteristics, which highlights the importance of comprehensive studies to better understand venom variability.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extente0253050pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleFrom birth to adulthood: an analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stagespt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1371/journal.pone.0253050pt_BR
dc.identifier.urlhttps://doi.org/10.1371/journal.pone.0253050pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.identifier.citationvolume16pt_BR
dc.identifier.citationissue6pt_BR
dc.subject.keywordVenomspt_BR
dc.subject.keywordSnakespt_BR
dc.subject.keywordPhospholipasespt_BR
dc.subject.keywordHemorrhagept_BR
dc.subject.keywordSerine proteasespt_BR
dc.subject.keywordMetalloproteasespt_BR
dc.subject.keywordNeonatespt_BR
dc.subject.keywordReversed-phase high performance liquid chromatographypt_BR
dc.relation.ispartofabbreviatedPloS Onept_BR
dc.identifier.citationabntv. 16, n. 6, e0253050, jun. 2021pt_BR
dc.identifier.citationvancouver2021 June;16(6):e0253050pt_BR
dc.contributor.butantanHatakeyama, Daniela Miki|:Aluno|:Lab. Herpetologia|:PrimeiroAutorpt_BR
dc.contributor.butantanTasima, Lidia Jorge|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanFabri, Caroline|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanStuginski, Daniel Rodrigues|:Técnico|:Lab. Herpetologiapt_BR
dc.contributor.butantanSant'anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦1736737pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25899-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25899-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/20106-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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