Catalytically active phospholipase A2 myotoxin from Crotalus durissus terrificus induces proliferation and differentiation of myoblasts dependent on prostaglandins produced by both COX-1 and COX-2 pathways

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dc.contributorCENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.authorSilva, Nadine C.pt_BR
dc.contributor.authorGómez, Angela María Alvarezpt_BR
dc.contributor.authorDe Ocesano-Pereira, Carlospt_BR
dc.contributor.authorFortes-Dias, Consuelo L.pt_BR
dc.contributor.authorMoreira, Vanessapt_BR
dc.date.accessioned2021-08-03T19:47:50Z-
dc.date.available2021-08-03T19:47:50Z-
dc.date.issued2021pt_BR
dc.identifier.citationSilva NC., Gómes AMA, DeOcesano-Pereira C, Fortes-Dias CL., Moreira V. Catalytically active phospholipase A2 myotoxin from Crotalus durissus terrificus induces proliferation and differentiation of myoblasts dependent on prostaglandins produced by both COX-1 and COX-2 pathways. Int. J. Biol. Macromol.. 2021 Sept;187:603-613. doi:10.1016/j.ijbiomac.2021.07.121.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3907-
dc.description.abstractAlthough crotoxin B (CB) is a well-established catalytically active secretory phospholipase A2 group IIA (sPLA2-IIA) myotoxin, we investigated its potential stimulatory effect on myogenesis with the involvement of prostaglandins (PGs) produced by cyclooxygenase (COX)-1 and -2 pathways. Myoblast C2C12 were cultured in proliferation or commitment protocols and incubated with CB followed by lumiracoxib (selective COX-2 inhibitor) or valeryl salicylate (selective COX-1 inhibitor) and subjected to analysis of PG release, cell proliferation and activation of myogenic regulatory factors (MRFs). Our data showed that CB in non-cytotoxic concentrations induces an increase of COX-2 protein expression and stimulates the activity of both COX isoforms to produce PGE2, PGD2 and 15d-PGJ2. CB induced an increase in the proliferation of C2C12 myoblast cells dependent on PGs from both COX-1 and COX-2 pathways. In addition, CB stimulated the activity of Pax7, MyoD, Myf5 and myogenin in proliferated cells. Otherwise, CB increased myogenin activity but not MyoD in committed cells. Our findings evidence the role of COX-1- and COX-2-derived PGs in modulating CB-induced activation of MRFs. This study contributes to the knowledge that CB promote early myogenic events via regulatory mechanisms on PG-dependent COX pathways, showing new concepts about the effect of sPLA2-IIA in skeletal muscle repair.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent603-613pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofInternational Journal of Biological Macromoleculespt_BR
dc.rightsRestricted accesspt_BR
dc.titleCatalytically active phospholipase A2 myotoxin from Crotalus durissus terrificus induces proliferation and differentiation of myoblasts dependent on prostaglandins produced by both COX-1 and COX-2 pathwayspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.ijbiomac.2021.07.121pt_BR
dc.identifier.urlttps://doi.org/10.1016/j.ijbiomac.2021.07.121pt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(FUNED) Fundação Ezequiel Diaspt_BR
dc.identifier.citationvolume187pt_BR
dc.subject.keywordMyotoxic sPLA2-IIApt_BR
dc.subject.keywordProstaglandispt_BR
dc.subject.keywordMyogenesispt_BR
dc.relation.ispartofabbreviatedInt. J. Biol. Macromol.pt_BR
dc.identifier.citationabntv. 187, 603-613, set. 2021pt_BR
dc.identifier.citationvancouver2021 Sept;187:603-613pt_BR
dc.contributor.butantanGómes, Angela Maria Alvarez|:Desvinculado|:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.butantanDe Ocesano-Pereira, Carlos|:Desvinculado|:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/25437-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/15107-6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/10937-3pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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