Staphylococcus aureus protection-related type 3 cell-mediated immune response elicited by recombinant proteins and GM-CSF DNA vaccine

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dc.contributor(LG) Laboratório de Genéticapt_BR
dc.contributor.authorSantos, Kamila R.pt_BR
dc.contributor.authorSouza, Fernando N.pt_BR
dc.contributor.authorRamos-Sanchez, Eduardo M.pt_BR
dc.contributor.authorBatista, Camila F.pt_BR
dc.contributor.authorReis, Luiza C.pt_BR
dc.contributor.authorFotoran, Wesley F.pt_BR
dc.contributor.authorHeinemann, Marcos B.pt_BR
dc.contributor.authorGoto, Hiropt_BR
dc.contributor.authorGidlund, Magnuspt_BR
dc.contributor.authorCunha, Adriano F.pt_BR
dc.contributor.authorFaria, Angélica Rosapt_BR
dc.contributor.authorAndrade, Hélida M.pt_BR
dc.contributor.authorLage, Andrey P.pt_BR
dc.contributor.authorCerqueira, Mônica M. O. P.pt_BR
dc.contributor.authorLibera, Alice M. M. P. Dellapt_BR
dc.date.accessioned2021-09-15T16:09:06Z-
dc.date.available2021-09-15T16:09:06Z-
dc.date.issued2021pt_BR
dc.identifier.citationSantos KR., Souza FN., Ramos-Sanchez EM., Batista CF., Reis LC., Fotoran WF., et al. Staphylococcus aureus protection-related type 3 cell-mediated immune response elicited by recombinant proteins and GM-CSF DNA vaccine. Vaccines. 2021 Aug;9(8):899. doi:10.3390/vaccines9080899.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3931-
dc.description.abstractStaphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and cysteinyl-tRNA synthetase (CTS) recombinant proteins; and G4: SAS+SDD+CTS plus GM-CSF DNA vaccine. The lymphocyte subpopulations, and the intracellular interleukin-17A (IL-17A) and interferon-γ production in the draining lymph node cells were immunophenotyped by flow cytometry. The immunophenotyping and lymphocyte proliferation was determined in spleen cells cultured with and without S. aureus stimulus. Immunization with S. aureus recombinant proteins generated memory cells in draining lymph nodes. Immunization with the three recombinant proteins plus GM-CSF DNA led to an increase in the percentage of IL-17A+ cells among overall CD44+ (memory), T CD4+, CD4+ T CD44+ CD27−, γδ TCR, γδ TCR+ CD44+ CD27+, and TCRVγ4+ cells. Vaccination with S. aureus recombinant proteins associated with GM-CSF DNA vaccine downregulated TH2 immunity. Immunization with the three recombinant proteins plus the GM-CSF DNA led to a proliferation of overall memory T, CD4+, and CD4+ TEM cells upon S. aureus stimulus. This approach fostered type 3 immunity, suggesting the development of a protective immune response against S. aureus.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent899pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofVaccinespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleStaphylococcus aureus protection-related type 3 cell-mediated immune response elicited by recombinant proteins and GM-CSF DNA vaccinept_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/vaccines9080899pt_BR
dc.identifier.urlhttps://doi.org/10.3390/vaccines9080899pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(UFPB) Universidade Federal da Paraíbapt_BR
dc.contributor.external(UNTRM) Universidad Nacional Toribio Rodríguez de Mendoza de Amazonaspt_BR
dc.contributor.external(UFMG) Universidade Federal de Minas Geraispt_BR
dc.contributor.external(UNIFAL-MG) Universidade Federal de Alfenaspt_BR
dc.identifier.citationvolume9pt_BR
dc.identifier.citationissue8pt_BR
dc.subject.keywordvaccinept_BR
dc.subject.keywordStaphylococcus aureuspt_BR
dc.subject.keywordT cell responsept_BR
dc.subject.keywordmastitispt_BR
dc.subject.keywordbovinept_BR
dc.relation.ispartofabbreviatedVaccinespt_BR
dc.identifier.citationabntv. 9, n. 8, 899, ago. 2021pt_BR
dc.identifier.citationvancouver2021 Aug;9(8):899pt_BR
dc.contributor.butantanFotoran, Wesley F.|:Desvinculado|:Lab. Genéticapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/23572-3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/23189-4pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.grantfulltextopen-
item.languageiso639-1English-
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