Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia

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dc.contributor(LBI) Laboratório de Imunoquímicapt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributorCeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.authorEto, Silas Fernandespt_BR
dc.contributor.authorFernandes, Dayanne Carlapt_BR
dc.contributor.authorBaldassi, Amanda Cristinapt_BR
dc.contributor.authorBalbuena, Thiago Santanapt_BR
dc.contributor.authorAlecrim, João Victor da Costapt_BR
dc.contributor.authorCarvalho, Fabíola Christian Almeida dept_BR
dc.contributor.authorLima, Carlapt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.contributor.authorPizauro, João Martinspt_BR
dc.date.accessioned2021-09-17T16:57:15Z-
dc.date.available2021-09-17T16:57:15Z-
dc.date.issued2021pt_BR
dc.identifier.citationEto SF, Fernandes DC, Baldassi AC, Balbuena TS, Alecrim JVC, Carvalho FCA, et al. Proteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microglia. Fish Shellfish Immunol.. 2021 Nov;118:34-50. doi:10.1016/j.fsi.2021.08.019.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3934-
dc.description.abstractGroup B Streptococcus (GBS) causes meningitis in neonates and Nile tilapia (Oreochromis niloticus). The molecular mechanisms regulating the intracellular survival of this pathogen in the host cell are complex and crucial for the progression of infection. Thus, we propose the use of GBS-infected Nile tilapia microglia as an in vitro model system simulating infection caused by homologous bacteria in humans. We used this model to evaluate the phagocytic activity, as well as the functional aspects of the capsular proteins A, B, C, and D and the major redox enzymes, and the synergistic role of mechanisms/proteins involved in blocking phagocytic process. We observed that in the intracellular phase, GBS showed enhanced synthesis of the polysaccharide capsule and used superoxide dismutase, thioredoxin, NADH oxidase, and alkyl hydroperoxide reductase to scavenge reactive oxygen species and reactive nitrogen species produced by the host cell. Furthermore, although these virulence mechanisms were effective during the initial hours of infection, they were not able to subvert microglial responses, which partially neutralized the infection. Altogether, our findings provided important information regarding the intracellular survival mechanisms of GBS and perspectives for the production of new drugs and vaccines, through the druggability analysis of specific proteins. In conclusion, tilapia microglia serve as a potent in vitro experimental model for the study of meningitis.pt_BR
dc.format.extent34-50pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFish & Shellfish Immunologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleProteomic analysis capsule synthesis and redox mechanisms in the intracellular survival of group B Streptococcus in fish microgliapt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.fsi.2021.08.019pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.fsi.2021.08.019pt_BR
dc.contributor.external(UFRR) Universidade Federal de Roraimapt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.identifier.citationvolume118pt_BR
dc.subject.keywordbrainpt_BR
dc.subject.keywordMeningitispt_BR
dc.subject.keywordProteomicspt_BR
dc.subject.keywordDruggabilitypt_BR
dc.subject.keywordvaccinept_BR
dc.relation.ispartofabbreviatedFish Shellfish Immunol.pt_BR
dc.identifier.citationabntv. 118, p. 34-50, nov. 2021pt_BR
dc.identifier.citationvancouver2021 Nov;118:34-50pt_BR
dc.contributor.butantanFernandes, Dayanne Carla|:Desvinculado|:Lab. Imunoquímica:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanLima, Carla|:Pesquisador:Docente PPGTox|:Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanLopes-Ferreira, Monica Valdyrce dos Anjos|:Pesquisador:Docente permanente PPGTOX|:Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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item.openairetypeArticle-
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