Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom

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dc.contributorLab. Fisiopatologiapt_BR
dc.contributorBiotério Centralpt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributorCeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributorLab. Bioquímicapt_BR
dc.contributorLab. Imunopatologiapt_BR
dc.contributor.authorThomazini, Camila Martospt_BR
dc.contributor.authorSachetto, Ana Teresa Azevedopt_BR
dc.contributor.authorAlbuquerque, Cynthia Zaccanini dept_BR
dc.contributor.authorMattaraia, Vânia Gomes de Mourapt_BR
dc.contributor.authorOliveira, Ana Karina dept_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.contributor.authorLebrun, Ivopt_BR
dc.contributor.authorBarbaro, Katia Cristinapt_BR
dc.contributor.authorSantoro, Marcelo Laramipt_BR
dc.date.accessioned2021-09-21T16:03:17Z-
dc.date.available2021-09-21T16:03:17Z-
dc.date.issued2021pt_BR
dc.identifier.citationThomazini CM, Sachetto ATA, Albuquerque CZ, Mattaraia VGM, Oliveira AK, Serrano SMT, et al. Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom. PLoS. Negl. Trop. Dis.. 2021 Sept;15(9):e0009715. doi:10.1371/journal.pntd.0009715.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3938-
dc.description.abstractPatients bitten by snakes consistently manifest a bleeding tendency, in which thrombocytopenia, consumption coagulopathy, mucous bleeding, and, more rarely, thrombotic microangiopathy, are observed. Von Willebrand factor (VWF) is required for primary hemostasis, and some venom proteins, such as botrocetin (a C-type lectin-like protein) and snake venom metalloproteinases (SVMP), disturb the normal interaction between platelets and VWF, possibly contributing to snakebite-induced bleedings. To understand the relationship among plasma VWF, platelets, botrocetin and SVMP from Bothrops jararaca snake venom (BjV) in the development of thrombocytopenia, we used (a) Wistar rats injected s.c. with BjV preincubated with anti-botrocetin antibodies (ABA) and/or Na2-EDTA (a SVMP inhibitor), and (b) VWF knockout mice (Vwf-/-) injected with BjV. Under all conditions, BjV induced a rapid and intense thrombocytopenia. In rats, BjV alone reduced the levels of VWF:Ag, VWF:CB, high molecular weight multimers of VWF, ADAMTS13 activity, and factor VIII. Moreover, VWF:Ag levels in rats that received BjV preincubated with Na2-EDTA and/or ABA tended to recover faster. In mice, BjV caused thrombocytopenia in both Vwf-/- and C57BL/6 (background control) strains, and VWF:Ag levels tended to decrease in C57BL/6, demonstrating that thrombocytopenia was independent of the presence of plasma VWF. These findings showed that botrocetin present in BjV failed to affect the extent or the time course of thrombocytopenia induced by envenomation, but it contributed to decrease the levels and function of plasma VWF. Thus, VWF alterations during B. jararaca envenomation are an ancillary event, and not the main mechanism leading to decreased platelet counts.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extente0009715pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPlos Neglected Tropical Diseasespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleInvolvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venompt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1371/journal.pntd.0009715pt_BR
dc.identifier.urlhttps://doi.org/10.1371/journal.pntd.0009715pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume15pt_BR
dc.identifier.citationissue9pt_BR
dc.subject.keywordThrombocytopeniapt_BR
dc.subject.keywordPlateletspt_BR
dc.subject.keywordvenomspt_BR
dc.subject.keywordsnakebitept_BR
dc.subject.keywordhemorrhagept_BR
dc.subject.keywordtoxinspt_BR
dc.subject.keywordBlood plasmapt_BR
dc.subject.keywordMouse modelspt_BR
dc.relation.ispartofabbreviatedPLoS. Negl. Trop. Dis.pt_BR
dc.identifier.citationabntv. 15, n. 9, e0009715, set. 2021pt_BR
dc.identifier.citationvancouver2021 Sept;15(9):e0009715pt_BR
dc.contributor.butantanThomazini, Camila Martos|:Desvinculado|:Lab. Fisiopatologia|:PrimeiroAutorpt_BR
dc.contributor.butantanSachetto, Ana Teresa Azevedo|:Desvinculado|:Lab. Fisiopatologiapt_BR
dc.contributor.butantanAlbuquerque, Cynthia Zaccanini de|:Outros|:Biotério Centralpt_BR
dc.contributor.butantanMattaraia, Vânia Gomes de Moura|:Pesquisador|:Biotério Centralpt_BR
dc.contributor.butantanOliveira, Ana Karina de|:Desvinculado|:Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente Permanente PPGTox|:Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.butantanLebrun, Ivo|:Pesquisador:Docente Permanente PPGTox|:Lab. Bioquímicapt_BR
dc.contributor.butantanBarbaro, Katia Cristina|:Pesquisador|:Lab. Imunopatologiapt_BR
dc.contributor.butantanSantoro, Marcelo Larami|:Pesquisador|:Lab. Fisiopatologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/25177-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/20239-8pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/26015-8pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/07618-6pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦305245/2015-5pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦312469/2018-7pt_BR
dc.sponsorship.butantanFundação Butantan¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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