Antimicrobial peptidomes of Bothrops atrox and Bothrops jararacussu snake venoms

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dc.contributorLab. Bioquímicapt_BR
dc.contributor.authorCaldeira, Cleópatra Alves da Silvapt_BR
dc.contributor.authorDiniz-Sousa, Rafaelapt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorSantos, Ana Paula Azevedo dospt_BR
dc.contributor.authorTeles, Carolina Bioni Garciapt_BR
dc.contributor.authorMatos, Najla Benevidespt_BR
dc.contributor.authorSilva, Saulo Luís dapt_BR
dc.contributor.authorStabeli, Rodrigo Guerinopt_BR
dc.contributor.authorCamperi, Silvia Andreapt_BR
dc.contributor.authorSoares, Andreimar Martinpt_BR
dc.contributor.authorCalderon, Leonardo de Azevedopt_BR
dc.date.accessioned2021-09-22T15:35:28Z-
dc.date.available2021-09-22T15:35:28Z-
dc.date.issued2021pt_BR
dc.identifier.citationCaldeira CAS, Diniz-Sousa R, Pimenta DC, Santos APA, Teles CBG, Matos NB, et al. Antimicrobial peptidomes of Bothrops atrox and Bothrops jararacussu snake venoms. Amino Acids. 2021 Oct;53:1635–1648. doi:10.1007/s00726-021-03055-y.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3939-
dc.description.abstractThe worrisome emergence of pathogens resistant to conventional drugs has stimulated the search for new classes of antimicrobial and antiparasitic agents from natural sources. Antimicrobial peptides (AMPs), acting through mechanisms that do not rely on the interaction with a specific receptor, provide new possibilities for the development of drugs against resistant organisms. This study sought to purify and proteomically characterize the antimicrobial and antiparasitic peptidomes of B. atrox and B. jararacussu snake venoms against Gram-positive (Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus—MRSA), Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, and the protozoan parasites Leishmania amazonensis and Plasmodium falciparum (clone W2, resistant to chloroquine). To this end, B. atrox and B. jararacussu venom peptides were purified by combination of 3 kDa cut-off Amicon® ultracentrifugal filters and reverse-phase high-performance liquid chromatography, and then identified by electrospray-ionization Ion-Trap/Time-of-Flight mass spectrometry. Fourteen distinct peptides, with masses ranging from 443.17 to 1383.73 Da and primary structure between 3 and 13 amino acid residues, were sequenced. Among them, 13 contained unique sequences, including 4 novel bradykinin-potentiating-like peptides (BPPs), and a snake venom metalloproteinase tripeptide inhibitor (SVMPi). Although commonly found in Viperidae venoms, except for Bax-12, the BPPs and SVMPi here reported had not been described in B. atrox and B. jararacussu venoms. Among the novel peptides, some exhibited bactericidal activity towards P. aeruginosa and S. aureus, had low hemolytic effect, and were devoid of antiparasitic activity. The identified novel antimicrobial peptides may be relevant in the development of new drugs for the management of multidrug-resistant Gram-negative and Gram-positive bacteria.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(INCT-FCx) Instituto Nacional de Ciência e Tecnologia de Fluidos Complexospt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPERO) Fundação Rondônia de Amparo ao Desenvolvimento das Ações Científicas e Tecnológicas e à Pesquisa do Estado de Rondôniapt_BR
dc.description.sponsorship(ANPCyT) Agencia Nacional de Promoción Científica y Tecnológicapt_BR
dc.description.sponsorship(FINEP) Financiadora de Estudos e Projetospt_BR
dc.description.sponsorship(CONICET) Consejo Nacional de Investigaciones Científicas y Técnicaspt_BR
dc.format.extent1635–1648pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofAmino Acidspt_BR
dc.rightsRestricted accesspt_BR
dc.titleAntimicrobial peptidomes of Bothrops atrox and Bothrops jararacussu snake venomspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1007/s00726-021-03055-ypt_BR
dc.identifier.urlhttps://doi.org/10.1007/s00726-021-03055-ypt_BR
dc.contributor.external(Fiocruz Rôndonia) Fundação Oswaldo Cruz de Rondôniapt_BR
dc.contributor.externalPrograma de Pós-Graduação em Biodiversidade e Biotecnologia - Rede Bionortept_BR
dc.contributor.external(UNIR) Universidade Federal de Rondôniapt_BR
dc.contributor.external(UNISL) Centro Universitário São Lucaspt_BR
dc.contributor.external(CEPEM) Centro de Pesquisa em Medicina Tropicalpt_BR
dc.contributor.externalUniversity of Cuencapt_BR
dc.contributor.externalUniversidade do Portopt_BR
dc.contributor.external(UFSCar) Universidade Federal de São Carlospt_BR
dc.contributor.external(UBA) Universidad de Buenos Airespt_BR
dc.contributor.external(FFyB) Facultad de Farmacia y Bioquimicapt_BR
dc.contributor.external(FIMCA) Centro Universitário Aparício Carvalhopt_BR
dc.contributor.external(FIOCRUZ) Fundação Oswaldo Cruzpt_BR
dc.identifier.citationvolume53pt_BR
dc.subject.keywordSnake venom peptidomept_BR
dc.subject.keywordBothrops atroxpt_BR
dc.subject.keywordBothrops jararacussupt_BR
dc.subject.keywordPeptidomicspt_BR
dc.subject.keywordantimicrobial peptidept_BR
dc.relation.ispartofabbreviatedAmino Acidspt_BR
dc.identifier.citationabntv. 53, p. 1635–1648, out. 2021pt_BR
dc.identifier.citationvancouver2021 Oct;53:1635–1648pt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente permanente PPGTOX|:Lab. Bioquímicapt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦406385/2018pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦01974/2019-5pt_BR
dc.sponsorship.butantanInstituto Nacional de Ciência e Tecnologia de Epidemiologia da Amazônia Ocidental (INCT-EPIAMO)¦¦pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantanFundação Rondônia de Amparo ao Desenvolvimento das Ações Científicas e Tecnológicas e à Pesquisa do Estado de Rondônia (FAPERO)¦¦pt_BR
dc.sponsorship.butantanAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT)¦¦pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦01.12.0450.0pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦01.09.0278.04pt_BR
dc.sponsorship.butantanConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.languageiso639-1English-
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