Avaliação do crescimento de células de Vero em sistema pseudo agitado com microcarregadores Cytodex 1

Abstract

Vero cells are used to develop viral vaccines approved for human use, thus including a vaccine against the SARS­CoV­2 virus. Our objective was to establish a production protocol for this cultivation condition. Cells grown in spinner flask were tested at different concentrations. The μmax values allow us to observe that the best performance was with the assay whose value was 0.0334 h . Cell duplication occurred at 1=37.47 h, 2=21.15 h and 3=22.29 h. The concentration and quality of the inoculum are crucial for the performance of cells when cultivated in a pseudo­stirred system with microcarriers