Robust immune response induced by Schistosoma mansoni TSP-2 antigen coupled to bacterial outer membrane vesicles

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dc.contributor(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributorLab. Imunopatologiapt_BR
dc.contributorPrograma de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributor.authorBarbosa, Mayra Mara Ferraript_BR
dc.contributor.authorKanno, Alex Issamupt_BR
dc.contributor.authorBarazzone, Giovana Cappiopt_BR
dc.contributor.authorRodríguez, Duniapt_BR
dc.contributor.authorPancakova, Violetapt_BR
dc.contributor.authorTrentini, Monalisa Martinspt_BR
dc.contributor.authorFaquim Mauro, Eliana Limapt_BR
dc.contributor.authorFreitas, Amanda Pereira dept_BR
dc.contributor.authorKhouri, Mariana Ipt_BR
dc.contributor.authorLobo-Silva, Jessicapt_BR
dc.contributor.authorGonçalves, Viviane Maimonipt_BR
dc.contributor.authorSchenkman, Rocilda Perazzini Furtadopt_BR
dc.contributor.authorTanizaki, Martha Massakopt_BR
dc.contributor.authorBoraschi, Dianapt_BR
dc.contributor.authorMalley, Richardpt_BR
dc.contributor.authorFarias, Leonardo Ppt_BR
dc.contributor.authorLeite, Luciana Cezar de Cerqueirapt_BR
dc.identifier.citationBarbosa MMF, Kanno AI, Rodríguez D, Pancakova V, Trentini MM, Faquim Mauro EL, et al. Robust immune response induced by Schistosoma mansoni TSP-2 antigen coupled to bacterial outer membrane vesicles. Int J Nanomedicine. 2021 Oct;2021(16):7153—7168. doi:10.2147/IJN.S315786.pt_BR
dc.description.abstractPurpose: The use of adjuvants can significantly strengthen a vaccine’s efficacy. We sought to explore the immunization efficacy of bacterial outer membrane vesicles (OMVs) displaying the Schistosoma mansoni antigen, SmTSP-2, through a biotin-rhizavidin coupling approach. The rationale is to exploit the nanoparticulate structure and the adjuvant properties of OMVs to induce a robust antigen-specific immune response, in light of developing new vaccines against S. mansoni. Materials and Methods: OMVs were obtained from Neisseria lactamica and conjugated with biotin. The recombinant SmTSP-2 in fusion with the biotin-binding protein rhizavidin (rRzvSmTSP-2) was produced in E. coli and coupled to biotinylated OMVs to generate an OMV complex displaying SmTSP-2 on the membrane surface (OMV:rSmTSP-2). Transmission electron microscopy (TEM) and dynamic light scattering analysis were used to determine particle charge and size. The immunogenicity of the vaccine complex was evaluated in C57BL/6 mice. Results: The rRzvSmTSP-2 protein was successfully coupled to biotinylated OMVs and purified by size-exclusion chromatography. The OMV:rSmTSP-2 nanoparticles showed an average size of 200 nm, with zeta potential around – 28 mV. Mouse Bone Marrow Dendritic Cells were activated by the nanoparticles as determined by increased expression of the co-stimulatory molecules CD40 and CD86, and the proinflammatory cytokines (TNF-α, IL-6 and IL-12) or IL-10. Splenocytes of mice immunized with OMV:rSmTSP-2 nanoparticles reacted to an in vitro challenge with SmTSP-2 with an increased production of IL-6, IL-10 and IL-17 and displayed a higher number of CD4+ and CD8+ T lymphocytes expressing IFN-γ, IL-4 and IL-2, compared to mice immunized with the antigen alone. Immunization of mice with OMV:rSmTSP-2 induced a 100-fold increase in specific anti-SmTSP-2 IgG antibody titers, as compared to the group receiving the recombinant rSmTSP-2 protein alone or even co-administered with unconjugated OMV. Conclusion: Our results demonstrate that the SmTSP-2 antigen coupled with OMVs is highly immunogenic in mice, supporting the potential effectiveness of this platform for improved antigen delivery in novel vaccine strategies.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CNR) Consiglio Nazionale delle Ricerchept_BR
dc.relation.ispartofInternational Journal of Nanomedicinept_BR
dc.rightsOpen accesspt_BR
dc.titleRobust immune response induced by Schistosoma mansoni TSP-2 antigen coupled to bacterial outer membrane vesiclespt_BR
dc.rights.licenseCC BY-NCpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.externalUniversité Claude Bernard Lyon 1pt_BR
dc.contributor.externalInstituto Gonçalo Moniz (IGM)pt_BR
dc.contributor.external(FIOCRUZ) Fundação Oswaldo Cruzpt_BR
dc.contributor.externalIstituto di Biochimica e Biologia Cellulare (IBBC)pt_BR
dc.contributor.externalStazione Zoologica Anton Dohrnpt_BR
dc.contributor.externalShenzhen Institute of Advanced Technologies (SIAT)pt_BR
dc.contributor.external(CAS) Chinese Academy of Sciencespt_BR
dc.contributor.externalBoston Children's Hospitalpt_BR
dc.subject.keywordSchistosoma mansonipt_BR
dc.subject.keywordTSP-2 antigenpt_BR
dc.subject.keywordouter membrane vesiclespt_BR
dc.subject.keywordbiotin-avidin couplingpt_BR
dc.relation.ispartofabbreviatedInt J Nanomedicinept_BR
dc.identifier.citationabntv. 2021, n. 16, p. 7153—7168, out. 2021pt_BR
dc.identifier.citationvancouver2021 Oct;2021(16):7153—7168pt_BR
dc.contributor.butantanBarbosa, Mayra Mara Ferrari|:Desvinculado|:(LDV) Lab. de Desenvolvimento de Vacinas|:PrimeiroAutorpt_BR
dc.contributor.butantanKanno, Alex Issamu|:Técnico|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanBarazzone, Giovana Cappio|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanRodríguez, Dunia|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanPancakova, Violeta|:Desvinculado|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanFaquim Mauro, Eliana Lima|:Pesquisador|:Lab. Imunopatologiapt_BR
dc.contributor.butantanFreitas, Amanda Pereira de|:Aluno Egresso|:Lab. Imunopatologia|:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributor.butantanGonçalves, Viviane Maimoni|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanSchenkman, Rocilda Perazzini Furtado|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanTanizaki, Martha Massako|:Desvinculado|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanLeite, Luciana Cezar de Cerqueira|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinas|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/24632-6pt_BR
dc.sponsorship.butantanFundação Butantan¦¦pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦160861/2017-9pt_BR
dc.sponsorship.butantan(CNR) Consiglio Nazionale delle Ricerche¦¦pt_BR
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