Inhibition of severe acute respiratory syndrome coronavirus 2 replication by hypertonic saline solution in lung and kidney epithelial cells

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dc.contributor(LV) Laboratório de Virologiapt_BR
dc.contributor.authorMachado, Rafael R. G.pt_BR
dc.contributor.authorGlaser, Talitapt_BR
dc.contributor.authorAraujo, Danielle B.pt_BR
dc.contributor.authorPetiz, Lyvia Lintzmaierpt_BR
dc.contributor.authorOliveira, Danielle Bruna Leal dept_BR
dc.contributor.authorDurigon, Giuliana S.pt_BR
dc.contributor.authorLeal, Alessandra L.pt_BR
dc.contributor.authorPinho, João Renato R.pt_BR
dc.contributor.authorFerreira, Luis C. S.pt_BR
dc.contributor.authorUlrich, Henningpt_BR
dc.contributor.authorDurigon, Edison L.pt_BR
dc.contributor.authorGuzzo, Cristiane Rodriguespt_BR
dc.date.accessioned2021-11-24T20:16:08Z-
dc.date.available2021-11-24T20:16:08Z-
dc.date.issued2021pt_BR
dc.identifier.citationMachado RR.G., Glaser T, Araujo DB., Petiz LL, Oliveira DBL, Durigon GS., et al. Inhibition of severe acute respiratory syndrome coronavirus 2 replication by hypertonic saline solution in lung and kidney epithelial cells. ACS Pharmacol. Transl. Sci. 2021 Sept;4(5):1514–1527. doi:10.1021/acsptsci.1c00080.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3992-
dc.description.abstractAn unprecedented global health crisis has been caused by a new virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We performed experiments to test if a hypertonic saline solution was capable of inhibiting virus replication. Our data show that 1.2% NaCl inhibited virus replication by 90%, achieving 100% of inhibition at 1.5% in the nonhuman primate kidney cell line Vero, and 1.1% of NaCl was sufficient to inhibit the virus replication by 88% in human epithelial lung cell line Calu-3. Furthermore, our results indicate that the inhibition is due to an intracellular mechanism and not to the dissociation of the spike SARS-CoV-2 protein and its human receptor. NaCl depolarizes the plasma membrane causing a low energy state (high ADP/ATP concentration ratio) without impairing mitochondrial function, supposedly associated with the inhibition of the SARS-CoV-2 life cycle. Membrane depolarization and intracellular energy deprivation are possible mechanisms by which the hypertonic saline solution efficiently prevents virus replication in vitro assays.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(MCT) Rede Víruspt_BR
dc.format.extent1514–1527pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofACS Pharmacology & Translational Sciencept_BR
dc.rightsRestricted accesspt_BR
dc.titleInhibition of severe acute respiratory syndrome coronavirus 2 replication by hypertonic saline solution in lung and kidney epithelial cellspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1021/acsptsci.1c00080pt_BR
dc.identifier.urlhttps://doi.org/10.1021/acsptsci.1c00080pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.externalHospital Israelita Albert Einsteinpt_BR
dc.contributor.externalFundação Hospital de Clínicas Gaspar Viannapt_BR
dc.identifier.citationvolume4pt_BR
dc.identifier.citationissue5pt_BR
dc.subject.keywordSARS-CoV-2pt_BR
dc.subject.keywordNaClpt_BR
dc.subject.keywordvirus inhibitionpt_BR
dc.subject.keywordcell depolarizationpt_BR
dc.subject.keywordATPpt_BR
dc.relation.ispartofabbreviatedACS Pharmacol. Transl. Scipt_BR
dc.identifier.citationabntv. 5, n. 4, p. 1514–1527, set. 2021pt_BR
dc.identifier.citationvancouver2021 Sept;4(5):1514–1527pt_BR
dc.contributor.butantanOliveira, Danielle Bruna Leal de|:Desvinculado|:Lab. Virologiapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/07366-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/00195-2pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2020/04680-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/24769-2pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2020/06409-1pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/20045-7pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦88887.131387/2016-00pt_BR
dc.sponsorship.butantan(MCT) Rede Vírus¦¦0459/20pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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