Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorSoria-Bustos, Jorgept_BR
dc.contributor.authorSaitz, Waleskapt_BR
dc.contributor.authorMedrano, Abrahampt_BR
dc.contributor.authorLara-Ochoa, Cristinapt_BR
dc.contributor.authorBennis, Zinebpt_BR
dc.contributor.authorMonteiro-Neto, Valeriopt_BR
dc.contributor.authorSantos, Camilla Itapary dospt_BR
dc.contributor.authorRodrigues, Josiaspt_BR
dc.contributor.authorHernandes, Rodrigo T.pt_BR
dc.contributor.authorYáñez, Jorge A.pt_BR
dc.contributor.authorTorres, Javierpt_BR
dc.contributor.authorNavarro-García, Fernandopt_BR
dc.contributor.authorMartínez-Laguna, Ygnaciopt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorMunhoz, Danielle Diaspt_BR
dc.contributor.authorCedillo, María L.pt_BR
dc.contributor.authorAres, Miguel Apt_BR
dc.contributor.authorCruz, Miguel A. De lapt_BR
dc.contributor.authorNataro, James P.pt_BR
dc.contributor.authorGirón, Jorge A.pt_BR
dc.identifier.citationSoria-Bustos J, Saitz W, Medrano A, Lara-Ochoa C, Bennis Z, Monteiro-Neto V, et al. Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli. Environ. Microbiol. 2021 Aug;in press. doi:10.1111/1462-2920.15737.pt_BR
dc.description.abstractThe interaction of enteroaggregative Escherichia coli (EAEC) strains with the colonic gut mucosa is characterized by the ability of the bacteria to form robust biofilms, to bind mucin, and induce a local inflammatory response. These events are mediated by a repertoire of five different aggregative adherence fimbriae variants (AAF/I-V) typically encoded on virulence plasmids. In this study, we report the production in EAEC strains of a new YehD fimbriae (YDF), which is encoded by the chromosomal gene cluster yehABCD, also present in most E. coli strains. Immuno-labelling of EAEC strain 042 with anti-AAF/II and anti-YDF antibodies demonstrated the presence of both AAF/II and YDF on the bacterial surface. We investigated the role of YDF in cell adherence, biofilm formation, colonization of spinach leaves, and induction of pro-inflammatory cytokines release. To this aim, we constructed yehD deletion mutants in different EAEC backgrounds (strains 17-2, 042, 55989, C1010, 278-1, J7) each harbouring one of the five AAFs. The effect of the YDF mutation was strain dependent and AAF independent as the lack of YDF had a different impact on the phenotypes manifested by the different EAECs tested. Expression of the yehABCD operon in a E. coli K12 ORN172 showed that YDF is important for biofilm formation but not for adherence to HeLa cells. Lastly, screening of pro-inflammatory cytokines in supernatants of Caco-2 cells infected with EAEC strains 042 and J7 and their isogenic ΔyehD mutants showed that these mutants were significantly defective in release of IL-8 and TNF-α. This study contributes to the understanding of the complex and diverse mechanisms of adherence of EAEC strains and identifies a new potential target for preventive measures of gastrointestinal illness caused by EAEC and other E. coli pathogroups.pt_BR
dc.description.sponsorship(NIAID) National Institute of Allergy and Infectious Diseasespt_BR
dc.relation.ispartofEnvironmental Microbiologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleRole of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia colipt_BR
dc.contributor.external(UVA) University of Virginiapt_BR
dc.contributor.external(IMSS) Instituto Mexicano del Seguro Socialpt_BR
dc.contributor.external(BUAP) Benemérita Universidad Autónoma de Pueblapt_BR
dc.contributor.external(UF) University of Floridapt_BR
dc.contributor.external(UFMA) Universidade Federal do Maranhãopt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.contributor.external(IPN) Instituto Politécnico Nacionalpt_BR
dc.relation.ispartofabbreviatedEnviron Microbiolpt_BR
dc.identifier.citationabntv. 24, n.3, p. 1035-1051, ago. 2021pt_BR
dc.identifier.citationvancouver2021 Aug; 24(3):1035-1051pt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanMunhoz, Danielle Dias|:Desvinculado|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantanNational Institute of Allergy and Infectious Diseases (NIAID)¦¦AI111189pt_BR
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