Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorSoria-Bustos, Jorgept_BR
dc.contributor.authorSaitz, Waleskapt_BR
dc.contributor.authorMedrano, Abrahampt_BR
dc.contributor.authorLara-Ochoa, Cristinapt_BR
dc.contributor.authorBennis, Zinebpt_BR
dc.contributor.authorMonteiro-Neto, Valeriopt_BR
dc.contributor.authorSantos, Camilla Itapary dospt_BR
dc.contributor.authorRodrigues, Josiaspt_BR
dc.contributor.authorHernandes, Rodrigo T.pt_BR
dc.contributor.authorYáñez, Jorge A.pt_BR
dc.contributor.authorTorres, Javierpt_BR
dc.contributor.authorNavarro-García, Fernandopt_BR
dc.contributor.authorMartínez-Laguna, Ygnaciopt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorMunhoz, Danielle Diaspt_BR
dc.contributor.authorCedillo, María L.pt_BR
dc.contributor.authorAres, Miguel Apt_BR
dc.contributor.authorCruz, Miguel A. De lapt_BR
dc.contributor.authorNataro, James P.pt_BR
dc.contributor.authorGirón, Jorge A.pt_BR
dc.date.accessioned2021-12-03T18:42:14Z-
dc.date.available2021-12-03T18:42:14Z-
dc.date.issued2021pt_BR
dc.identifier.citationSoria-Bustos J, Saitz W, Medrano A, Lara-Ochoa C, Bennis Z, Monteiro-Neto V, et al. Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli. Environ. Microbiol. 2021 Aug;in press. doi:10.1111/1462-2920.15737.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4002-
dc.description.abstractThe interaction of enteroaggregative Escherichia coli (EAEC) strains with the colonic gut mucosa is characterized by the ability of the bacteria to form robust biofilms, to bind mucin, and induce a local inflammatory response. These events are mediated by a repertoire of five different aggregative adherence fimbriae variants (AAF/I-V) typically encoded on virulence plasmids. In this study, we report the production in EAEC strains of a new YehD fimbriae (YDF), which is encoded by the chromosomal gene cluster yehABCD, also present in most E. coli strains. Immuno-labelling of EAEC strain 042 with anti-AAF/II and anti-YDF antibodies demonstrated the presence of both AAF/II and YDF on the bacterial surface. We investigated the role of YDF in cell adherence, biofilm formation, colonization of spinach leaves, and induction of pro-inflammatory cytokines release. To this aim, we constructed yehD deletion mutants in different EAEC backgrounds (strains 17-2, 042, 55989, C1010, 278-1, J7) each harbouring one of the five AAFs. The effect of the YDF mutation was strain dependent and AAF independent as the lack of YDF had a different impact on the phenotypes manifested by the different EAECs tested. Expression of the yehABCD operon in a E. coli K12 ORN172 showed that YDF is important for biofilm formation but not for adherence to HeLa cells. Lastly, screening of pro-inflammatory cytokines in supernatants of Caco-2 cells infected with EAEC strains 042 and J7 and their isogenic ΔyehD mutants showed that these mutants were significantly defective in release of IL-8 and TNF-α. This study contributes to the understanding of the complex and diverse mechanisms of adherence of EAEC strains and identifies a new potential target for preventive measures of gastrointestinal illness caused by EAEC and other E. coli pathogroups.pt_BR
dc.description.sponsorship(NIAID) National Institute of Allergy and Infectious Diseasespt_BR
dc.format.extent1035-1051pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofEnvironmental Microbiologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleRole of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia colipt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1111/1462-2920.15737pt_BR
dc.identifier.urlhttps://doi.org/10.1111/1462-2920.15737pt_BR
dc.contributor.external(UVA) University of Virginiapt_BR
dc.contributor.external(IMSS) Instituto Mexicano del Seguro Socialpt_BR
dc.contributor.external(BUAP) Benemérita Universidad Autónoma de Pueblapt_BR
dc.contributor.external(UF) University of Floridapt_BR
dc.contributor.external(UFMA) Universidade Federal do Maranhãopt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.contributor.external(IPN) Instituto Politécnico Nacionalpt_BR
dc.identifier.citationvolume24pt_BR
dc.identifier.citationissue3pt_BR
dc.relation.ispartofabbreviatedEnviron Microbiolpt_BR
dc.identifier.citationabntv. 24, n.3, p. 1035-1051, ago. 2021pt_BR
dc.identifier.citationvancouver2021 Aug; 24(3):1035-1051pt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanMunhoz, Danielle Dias|:Desvinculado|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantanNational Institute of Allergy and Infectious Diseases (NIAID)¦¦AI111189pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
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