Indoleamine 2,3-dioxygenase-1 expression is changed during bladder cancer cell invasion

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dc.contributorLab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorSantos, Hellen Joyce Sousa Pereirapt_BR
dc.contributor.authorMatheus, Luiz Henrique Gomespt_BR
dc.contributor.authorSilva, Alinept_BR
dc.contributor.authorDalmazzo, Stephanie Vaninpt_BR
dc.contributor.authorSantos, Andressa Assunçãopt_BR
dc.contributor.authorSantos, Letícia Rafaela Alves Rubenspt_BR
dc.contributor.authorSouza, Diego Motapt_BR
dc.contributor.authorReis, Sabrina Thalitapt_BR
dc.contributor.authorNascimento, Ivan Pereirapt_BR
dc.contributor.authorDellê, Humbertopt_BR
dc.identifier.citationSantos HJSP, Matheus LHG, Silva A, Dalmazzo SV, Santos AA, Santos LRAR, et al. Indoleamine 2,3-dioxygenase-1 expression is changed during bladder cancer cell invasion. Int. J. Tryptophan. Res.. 2022 Jan; 15:1-6. doi:10.1177/11786469211065612.pt_BR
dc.description.abstractThe severity of the bladder carcinoma (BC) is directly linked to cell invasion and metastasis. Indoleamine 2,3-dioxygenase-1 (IDO-1) is an INF-γ-induced immunomodulating enzyme that has been linked to the cancer cell invasiveness. Because IDO1 is variable among the tumors, we analyzed its expression in the BC invasion using BC mice models and cell culture. MB49 cells were orthotopically or ectopically inoculated in C57Bl6 mice to evaluate IDO1 by immunohistochemistry. For in vitro experiments, expression of IDO1 and INF-γ was evaluated in grade-1 (RT4) and in grade-3 (T24) BC cell lines. Invading and non-invading T24 cells were separated using the Matrigel/Transwell system, of which total RNA was extracted immediately or after 2 weeks of subculture. Finally, IDO1 was silenced in T24 cells to verify its role on cell invasiveness. In both animal models, IDO1 was differentially expressed between non-invading and invading cells. In cell culture, T24 cells expressed more IDO1 than RT4 cells, independently of the INF-γ expression. IDO1 was differentially expressed between non-invading and invading T24 cells, a difference that was lost by long-time subculture. IDO1 silencing resulted in diminished cell invasiveness. In conclusion, IDO1 expression is changed during bladder carcinoma invasion, playing an important role in this process.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartofInternational Journal of Tryptophan Researchpt_BR
dc.rightsOpen accesspt_BR
dc.titleIndoleamine 2,3-dioxygenase-1 expression is changed during bladder cancer cell invasionpt_BR
dc.title.alternativeA expressão da indoleamina 2,3-dioxigenase-1 é alterada durante a invasão das células cancerígenas da bexigapt_BR
dc.rights.licenseCC BY-NCpt_BR
dc.contributor.external(UNINOVE) Universidade Nove de Julhopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.subject.keywordBladder cancerpt_BR
dc.subject.keywordindoleamine 2,3-dioxygenasept_BR
dc.subject.keywordcancer invasivenesspt_BR
dc.relation.ispartofabbreviatedInt. J. Tryptophan. Res.pt_BR
dc.identifier.citationabntv. 15, p. 1-6, jan. 2022pt_BR
dc.identifier.citationvancouver2022 Jan; 15:1-6pt_BR
dc.contributor.butantanNascimento, Ivan Pereira|:Pesquisador|:Laboratório de Desenvolvimento de Vacinaspt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/04105-0pt_BR
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