Using crystallography tools to improve vaccine formulations

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dc.contributorCentro Bioindustrialpt_BR
dc.contributorLab. Imunogenéticapt_BR
dc.contributor(LV) Lab. Virologiapt_BR
dc.contributor(LBI) Lab. Imunoquímicapt_BR
dc.contributor.authorFantini, Márcia Carvalho de Abreupt_BR
dc.contributor.authorOliveira, Cristiano Luis Pintopt_BR
dc.contributor.authorLopes, José Luiz de Souzapt_BR
dc.contributor.authorMartins, Tereza da Silvapt_BR
dc.contributor.authorAkamatsu, Milena Apetitopt_BR
dc.contributor.authorTrezena, Aryene Góespt_BR
dc.contributor.authorDe Franco, Milene Tinopt_BR
dc.contributor.authorBotosso, Viviane Fongaropt_BR
dc.contributor.authorSant'anna, Osvaldo Augustopt_BR
dc.contributor.authorKardjilov, Nikolaypt_BR
dc.contributor.authorRasmussen, Martin Kjaerulfpt_BR
dc.contributor.authorBordallo, Heloísa Nunespt_BR
dc.date.accessioned2022-01-27T16:15:05Z-
dc.date.available2022-01-27T16:15:05Z-
dc.date.issued2022pt_BR
dc.identifier.citationFantini MCA, Oliveira CLP, Lopes JLS, Martins TS, Akamatsu MA, Trezena AG, et al. Using crystallography tools to improve vaccine formulations. IUCrJ. 2022 Jan;9(1):11-20. doi:10.1107/S205225252101071X.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4110-
dc.description.abstractThis article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer's patches in the small intestine to be reached and consequently enhancing immunity. Focusing on the pioneering research conducted at the Butantan Institute in Brazil, the optimization of the antigen encapsulation yield is reported, as well as their distribution inside the meso- and macroporous network of the porous silica. As the development of vaccines requires proper inclusion of antigens in the antibody cells, X-ray crystallography is one of the most commonly used techniques to unveil the structure of antibody-combining sites with protein antigens. Thus structural characterization and modelling of pure antigen structures, showing different dimensions, as well as their complexes, such as silica with encapsulated hepatitis B virus-like particles and diphtheria anatoxin, were performed using small-angle X-ray scattering, X-ray absorption spectroscopy, X-ray phase contrast tomography, and neutron and X-ray imaging. By combining crystallography with dynamic light scattering and transmission electron microscopy, a clearer picture of the proposed vaccine complexes is shown. Additionally, the stability of the immunogenic complex at different pH values and temperatures was checked and the efficacy of the proposed oral immunogenic complex was demonstrated. The latter was obtained by comparing the antibodies in mice with variable high and low antibody responses.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(DASTI) Danish Agency for Science, Technology and Innovationpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent11-20pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofIUCrJpt_BR
dc.rightsOpen accesspt_BR
dc.titleUsing crystallography tools to improve vaccine formulationspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1107/S205225252101071Xpt_BR
dc.identifier.urlhttps://doi.org/10.1107/S205225252101071Xpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(HZB) Helmholtz Center Berlin for Materials and Energypt_BR
dc.contributor.externalUniversity of Copenhagenpt_BR
dc.identifier.citationvolume9pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordoral vaccinespt_BR
dc.subject.keywordporous silicapt_BR
dc.subject.keywordSAXSpt_BR
dc.subject.keywordXASpt_BR
dc.subject.keywordimagingpt_BR
dc.relation.ispartofabbreviatedIUCrJpt_BR
dc.identifier.citationabntv. 9, n. 1, p. 11-20, jan. 2022pt_BR
dc.identifier.citationvancouver2022 Jan;9(1):11-20pt_BR
dc.contributor.butantanAkamatsu, Milena Apetito|:Pesquisador|:Centro Bioindustrialpt_BR
dc.contributor.butantanTrezena, Aryene Góes|:Pesquisador|:Lab. Imunogenéticapt_BR
dc.contributor.butantanDe Franco, Milene Tino|:Pesquisador|:Lab. Imunogenéticapt_BR
dc.contributor.butantanBotosso, Viviane Fongaro|:Pesquisador|:(LV) Lab. Virologiapt_BR
dc.contributor.butantanSant'anna, Osvaldo Augusto|:Pesquisador|:Lab. Imunoquímicapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/17844–8pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/19546–7pt_BR
dc.sponsorship.butantan(DASTI) Danish Agency for Science, Technology and Innovation¦¦5132-00054Bpt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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