A modified ELISA method to evaluate the interaction of schistosoma mansoni proteins with plasminogen


Publication type
Book chapter
Language
English
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Restricted access
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Abstract
An important aspect of host-pathogen interactions is the interference of secreted proteins with the fibrinolytic system. Herein, we describe a modified ELISA method used to evaluate the interaction of a recombinant Schistosoma mansoni protein with plasminogen (PLG). Using this protocol, we demonstrated that a secreted protein, recombinant venom allergen-like protein 18 (rSmVAL18) acts as a plasminogen receptor increasing its activation into plasmin in the presence of the urokinase-type plasminogen activator (uPA). PLG binding was determined by immobilizing human PLG in the plate and incubating with the recombinant protein; competitive binding with a lysine analog demonstrated the interaction of the protein lysine residues with PLG Kringle domains. To assess the activation of S. mansoni recombinant protein-bound PLG, the amidolytic activity of generated plasmin was measured using the d-Val-Leu-Lys 4-nitroanilide dihydrochloride substrate.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4153
Issue Date
2020

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