Crotamine cell-penetrating nanocarriers: cancer-targeting and potential biotechnological and/or medical applications

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dc.contributor(LG) Lab. Genéticapt_BR
dc.contributor.authorHayashi, Mirian Akemi Furuiept_BR
dc.contributor.authorCampeiro, Joana Darcpt_BR
dc.contributor.authorPorta, Lucas Carvalhopt_BR
dc.contributor.authorSzychowski, Brianpt_BR
dc.contributor.authorAlves, Wendel Andradept_BR
dc.contributor.authorOliveira, Eduardo Brandt dept_BR
dc.contributor.authorKerkis, Irinapt_BR
dc.contributor.authorDaniel, Marie-Christinept_BR
dc.contributor.authorKarpel, Richard L.pt_BR
dc.date.accessioned2022-02-10T16:45:44Z-
dc.date.available2022-02-10T16:45:44Z-
dc.date.issued2020pt_BR
dc.identifier.isbn978-1-0716-0318-5pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4163-
dc.description.abstractCrotamine is a basic, 42-residue polypeptide from snake venom that has been shown to possess cell-penetrating properties. Here we describe the preparation, purification, biochemical and biophysical analysis of venom-derived, recombinant, chemically synthesized, and fluorescent-labeled crotamine. We also describe the formation and characterization of crotamine–DNA and crotamine–RNA nanoparticles; and the delivery of these nanoparticles into cells and animals. Crotamine forms nanoparticles with a variety of DNA and RNA molecules, and crotamine–plasmid DNA nanoparticles are selectively delivered into actively proliferating cells in culture or in living organisms such as mice, Plasmodium, and worms. As such, these nanoparticles could form the basis for a nucleic acid drug-delivery system. We also describe here the design and characterization of crotamine-functionalized gold nanoparticles, and the delivery of these nanoparticles into cells. We also evaluated the viability of using the combination of crotamine with silica nanoparticles in animal models, aiming to provide slow delivery, and to decrease the crotamine doses needed for the biological effects. In addition, the efficacy of administering crotamine orally was also demonstrated.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent29 p.pt_BR
dc.language.isoEnglishpt_BR
dc.publisherHumanapt_BR
dc.relation.ispartofseries2118pt_BR
dc.rightsRestricted accesspt_BR
dc.titleCrotamine cell-penetrating nanocarriers: cancer-targeting and potential biotechnological and/or medical applicationspt_BR
dc.typeBook chapterpt_BR
dc.identifier.doi10.1007/978-1-0716-0319-2_5pt_BR
dc.identifier.urlhttps://doi.org/10.1007/978-1-0716-0319-2_5pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.contributor.externalUniversity of Marylandpt_BR
dc.contributor.external(UFABC) Universidade Federal do ABCpt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.publisher.cityNew Yorkpt_BR
dc.subject.keywordCell-penetrating peptidespt_BR
dc.subject.keywordAmphipathic peptidespt_BR
dc.subject.keywordProtein–DNA nanoparticlespt_BR
dc.identifier.citationabntv. 2118, 29 p., mar. 2020pt_BR
dc.identifier.citationvancouver2020 Mar; 2118:29pt_BR
dc.publisher.countryUnited Statespt_BR
dc.contributor.butantanKerkis, Irina|:Desvinculado:Lab. Genética|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.relation.ispartofbookMethods in Molecular Biologypt_BR
dc.contributor.externalcountryBrazilpt_BR
dc.contributor.externalcountryUnited Statespt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextnone-
item.languageiso639-1English-
item.openairetypeBook chapter-
item.fulltextSem Texto completo-
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