Following trypanosoma cruzi RPA-DNA interaction using fluorescent in situ hybridization coupled with immunofluorescence (FISH/IF)


Publication type
Book chapter
Language
English
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Abstract
Fluorescent in situ hybridization coupled with immunofluorescence (FISH/IF) is an assay that has been widely used to study DNA-protein interactions. The technique is based on the use of a fluorescent nucleic acid probe and fluorescent antibodies to reveal the localization of a DNA sequence and a specific protein in the cell. The interaction can be inferred by the quantification of the co-localization between the protein and the DNA. Here, we describe a detailed FISH/IF methodology that our group used to study RPA-telomere interaction in the pathogenic protozoa parasite Trypanosoma cruzi.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4219
Issue Date
2021

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