Specific gene silencing in Leptospira biflexa by RNA-guided catalytically inactive Cas9 (dCas9)

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dc.contributorLab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorFernandes, Luis Guilherme Vírgíliopt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Ollerpt_BR
dc.date.accessioned2022-02-24T12:53:59Z-
dc.date.available2022-02-24T12:53:59Z-
dc.date.issued2020pt_BR
dc.identifier.isbn978-1-0716-0458-8pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4229-
dc.description.abstractEasy, practical, and affordable gene silencing techniques are constantly progressing, and genetic tools such as TALEs, RNAi, and CRISPR/Cas9 have emerged as new techniques for understanding the basic biology and virulence mechanisms of pathogenic organisms, including bacteria. Here, we describe one-step targeted gene silencing in Leptospira biflexa by using plasmids expressing catalytically inactive Streptococcus pyogenes Cas9 (dCas9) and a single-guide RNA (sgRNA) capable of pairing to the coding strand of a desired gene.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent109-122 p.pt_BR
dc.language.isoPortuguesept_BR
dc.publisherHumanapt_BR
dc.relation.ispartofseries2134pt_BR
dc.rightsRestricted accesspt_BR
dc.titleSpecific gene silencing in Leptospira biflexa by RNA-guided catalytically inactive Cas9 (dCas9)pt_BR
dc.typeBook chapterpt_BR
dc.identifier.doi10.1007/978-1-0716-0459-5_10pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.subject.keywordSingle-guide RNApt_BR
dc.subject.keyworddCas9pt_BR
dc.subject.keywordGene silencingpt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordElectroporationpt_BR
dc.identifier.citationabntv. 2134, 109-122 p., jul. 2020pt_BR
dc.identifier.citationvancouver2020 Jul; 2134:109-122pt_BR
dc.publisher.countryUnited Statespt_BR
dc.contributor.butantanFernandes, Luis Guilherme Vírgílio|:Aluno|:Lab. de Desenvolvimento de Vacinas|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller|:Pesquisador|:Lab. de Desenvolvimento de Vacinas|:Autorpt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/50981-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/06731-8pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦301229/2017-1pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦441449/2014-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.relation.ispartofbookMethods in Molecular Biologypt_BR
dc.contributor.externalcountryBrazilpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextnone-
item.openairetypeBook chapter-
item.languageiso639-1Portuguese-
item.fulltextSem Texto completo-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-4851-0870-
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