Substrate capture assay using inactive Oligopeptidases to identify novel peptides


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Publication type
Book chapter
Language
English
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Restricted access
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Abstract
Researchers are always searching for novel biologically active molecules including peptides. With the improvement of equipment for electrospray mass spectrometry, it is now possible to identify hundreds of novel peptides in a single run. However, after identifying the peptide sequences it is expensive to synthesize all the peptides to perform biological activity assays. Here, we describe a substrate capture assay that uses inactive oligopeptidases to identify putative biologically active peptides in complexes peptide mixtures. This methodology can use any crude extracts of biological tissues or cells, with the advantage to introduce a filter (i.e., binding to an inactive oligopeptidase) as a prior step in screening to bioactive peptides.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4254
Issue Date
2018

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