Ação do campo elétrico alternado externo em células tumorais de melanoma e em fibroblastos normais

Abstract

Melanoma is a malignant melanocytic neoplasm that arises from melanocytes found in the skin, eyes and, to a lesser extent, other body tissues. Major advances in two classes of therapy have completely changed as strategies in the treatment of melanoma. However, despite advances in recent decades, patients with BRAF inhibition show disease progression during the first 1 to 2 years of therapy. Thus, new therapeutic approaches are required for the treatment of melanoma. From this problematic, the present work aims to evaluate the biological effects of the external application of 10V and 5V alternating electric field intensities (CEAE) at 200kHz, 1MHz and 2MHz in murine melanoma cells (B16F10) and normal human fibroblasts (FN1). For this purpose, in vitro tests were performed to verify the evaluation of the cytotoxic effect of CEAE in colorimetric tests for the determination of cell viability and LPO assay based on the formation of thiobarbituric acid reactive substances (TBA). The FN1 cells were less harmed by the effects of CEAE than B16F10 melanoma cells, suggesting a new therapeutic possibility for melanoma treatment, which will depend on further studies using CEAE, with more detail on cell kinetics and pathways death associated.