Differences in PLA2 Constitution Distinguish the Venom of Two Endemic Brazilian Mountain Lanceheads, Bothrops cotiara and Bothrops fonsecai

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dc.contributor(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributorLab. Imunopatologiapt_BR
dc.contributorLab. Coleções Zoológicaspt_BR
dc.contributor.authorNachtigall, Pedro Gabrielpt_BR
dc.contributor.authorFreitas-de-Sousa, Luciana Aparecidapt_BR
dc.contributor.authorMason, Andrew J.pt_BR
dc.contributor.authorMoura-da-Silva, Ana Mariapt_BR
dc.contributor.authorGrazziotin, Felipe Gobbipt_BR
dc.contributor.authorJunqueira-de-Azevedo, Inácio de Loiola Meirellespt_BR
dc.date.accessioned2022-05-02T17:18:22Z-
dc.date.available2022-05-02T17:18:22Z-
dc.date.issued2022pt_BR
dc.identifier.citationNachtigall PG, Freitas-de-Sousa LA, Mason AJ., Moura-da-Silva AM, Grazziotin FG, Junqueira-de-Azevedo ILM. Differences in PLA2 Constitution Distinguish the Venom of Two Endemic Brazilian Mountain Lanceheads, Bothrops cotiara and Bothrops fonsecai. Toxins. 2022 Mar; 14(4):237. doi:10.3390/toxins14040237.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4313-
dc.description.abstractInterspecific differences in snake venom compositions can result from distinct regulatory mechanisms acting in each species. However, comparative analyses focusing on identifying regulatory elements and patterns that led to distinct venom composition are still scarce. Among venomous snakes, Bothrops cotiara and Bothrops fonsecai represent ideal models to complement our understanding of the regulatory mechanisms of venom production. These recently diverged species share a similar specialized diet, habitat, and natural history, but each presents a distinct venom phenotype. Here, we integrated data from the venom gland transcriptome and miRNome and the venom proteome of B. fonsecai and B. cotiara to better understand the regulatory mechanisms that may be acting to produce differing venom compositions. We detected not only the presence of similar toxin isoforms in both species but also distinct expression profiles of phospholipases A2 (PLA2) and some snake venom metalloproteinases (SVMPs) and snake venom serine proteinases (SVSPs) isoforms. We found evidence of modular expression regulation of several toxin isoforms implicated in venom divergence and observed correlated expression of several transcription factors. We did not find strong evidence for miRNAs shaping interspecific divergence of the venom phenotypes, but we identified a subset of toxin isoforms whose final expression may be fine-tuned by specific miRNAs. Sequence analysis on orthologous toxins showed a high rate of substitutions between PLA2s, which indicates that these toxins may be under strong positive selection or represent paralogous toxins in these species. Our results support other recent studies in suggesting that gene regulation is a principal mode of venom evolution across recent timescales, especially among species with conserved ecotypes.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent237pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleDifferences in PLA2 Constitution Distinguish the Venom of Two Endemic Brazilian Mountain Lanceheads, Bothrops cotiara and Bothrops fonsecaipt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins14040237pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins14040237pt_BR
dc.contributor.externalOhio State Universitypt_BR
dc.identifier.citationvolume14pt_BR
dc.identifier.citationissue4pt_BR
dc.subject.keywordtranscriptomept_BR
dc.subject.keywordproteomept_BR
dc.subject.keywordvenompt_BR
dc.subject.keywordevolutionpt_BR
dc.subject.keywordgene expressionpt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 14, n. 4, p. 237, 2022pt_BR
dc.identifier.citationvancouver2022 Mar; 14(4):237pt_BR
dc.contributor.butantanNachtigall, Pedro Gabriel|:Aluno|:(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributor.butantanFreitas-de-Sousa, Luciana Aparecida|:Técnico|:Lab. Imunopatologiapt_BR
dc.contributor.butantanMoura-da-Silva, Ana Maria|:Pesquisador|:Lab. Imunopatologiapt_BR
dc.contributor.butantanGrazziotin, Felipe Gobbi|:Pesquisador|:Lab. Coleções Zoológicaspt_BR
dc.contributor.butantanJunqueira-de-Azevedo, Inácio de Loiola Meirelles|:Pesquisador|:(LETA) Lab. Toxinologia Aplicadapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/50127-5pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/26520-4pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextopen-
item.languageiso639-1English-
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