Proliferation and invasion of melanoma are suppressed by a plant protease inhibitor, leading to downregulation of survival/death-related proteins

Full metadata record
DC FieldValueLanguage
dc.contributor(CENTD) Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor(LDI) Lab. Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.authorBonturi, Camila Ramalhopt_BR
dc.contributor.authorSalu, Bruno Ramospt_BR
dc.contributor.authorBonazza, Camila Nimript_BR
dc.contributor.authorSinigaglia, Rita de Cassiapt_BR
dc.contributor.authorRodrigues, Tiagopt_BR
dc.contributor.authorFlores, Miryam Paola Alvarezpt_BR
dc.contributor.authorChudzinski-Tavassi, Ana Marisapt_BR
dc.contributor.authorOliva, Maria Luiza Vilelapt_BR
dc.date.accessioned2022-05-18T12:32:53Z-
dc.date.available2022-05-18T12:32:53Z-
dc.date.issued2022pt_BR
dc.identifier.citationBonturi CR, Salu BR, Bonazza CN, Sinigaglia RC, Rodrigues T, Flores MPA, et al. Proliferation and invasion of melanoma are suppressed by a plant protease inhibitor, leading to downregulation of survival/death-related proteins. Molecules. 2022 May; 27(9):2956. doi:10.3390/molecules27092956.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4345-
dc.description.abstractCell adhesion and migration are crucial for cancer progression and malignancy. Drugs available for the treatment of metastatic melanoma are expensive and unfit for certain patients. Therefore, there is still a need to identify new drugs that block tumor cell development. We investigated the effects of Enterolobium contortisiliquum trypsin inhibitor (EcTI), a protease inhibitor, on cell viability, cell migration, invasion, cell adhesion, and cell death (hallmarks of cancer) in vitro using human melanoma cells (SK-MEL-28 and CHL-1). Although EcTI did not affect non-tumor cells, it significantly inhibited the proliferation, migration, invasion, and adhesion of melanoma cells. Investigation of the underlying mechanisms revealed that EcTI triggered apoptosis and nuclear shrinkage, increased PI uptake, activated effector caspases-3/7, and produced reactive oxygen species (ROS). Furthermore, EcTI disrupted the mitochondrial membrane potential, altered calcium homeostasis, and modified proteins associated with survival and apoptosis/autophagy regulation. Acridine orange staining indicated acidic vesicular organelle formation upon EcTI treatment, demonstrating a cell death display. Electronic microscopy corroborated the apoptotic pattern by allowing the visualization of apoptotic bodies, mitochondrial cristae disorganization, and autophagic vesicles. Taken together, these results provide new insights into the anti-cancer properties of the natural EcTI protein, establishing it as a promising new therapeutic drug for use in melanoma treatment.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent2956pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofMoleculespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleProliferation and invasion of melanoma are suppressed by a plant protease inhibitor, leading to downregulation of survival/death-related proteinspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/molecules27092956pt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(UFABC) Universidade Federal do ABCpt_BR
dc.identifier.citationvolume27pt_BR
dc.identifier.citationissue9pt_BR
dc.subject.keywordEcTIpt_BR
dc.subject.keywordmelanomapt_BR
dc.subject.keywordmetastasispt_BR
dc.subject.keywordprotease inhibitorpt_BR
dc.subject.keywordskin cancerpt_BR
dc.relation.ispartofabbreviatedMoleculespt_BR
dc.identifier.citationabntv. 27, n. 9, 2956, mai. 2022pt_BR
dc.identifier.citationvancouver2022 May; 27(9):2956pt_BR
dc.contributor.butantanFlores, Miryam Paola Alvarez|:Técnico|:(CENTD) Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.butantanChudzinski-Tavassi, Ana Marisa|:Pesquisador|:(CENTD) Centro de Excelência para Descoberta de Alvos Moleculares: (LDI) (LDI) Lab. Desenvolvimento e Inovação Industrialpt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/06630-7pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/22243-9pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦302658/2021-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-7717-7013-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos


Files in This Item:

molecules-27-02956-v2.pdf
Description:
Size: 4.73 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons