p53 gene delivery via a recombinant Salmonella enterica Typhimurium leads to human bladder carcinoma cell death in vitro

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dc.contributor(LG) Lab. Genéticapt_BR
dc.contributor.authorJorge, Genesy Pérezpt_BR
dc.contributor.authorMódolo, Diego Grandopt_BR
dc.contributor.authorJaimes-Florez, Yessica Paolapt_BR
dc.contributor.authorFávaro, Wagner Josépt_BR
dc.contributor.authorJesus, Marcelo Bispo Dept_BR
dc.contributor.authorBrocchi, Marcelopt_BR
dc.date.accessioned2022-06-27T13:46:59Z-
dc.date.available2022-06-27T13:46:59Z-
dc.date.issued2022pt_BR
dc.identifier.citationJorge GP, Módolo DG, Jaimes-Florez YP, Fávaro WJ, Jesus MBD, Brocchi M. p53 gene delivery via a recombinant Salmonella enterica Typhimurium leads to human bladder carcinoma cell death in vitro. Lett. Appl. Microbiol. 2022 June;in press. doi:10.1111/lam.13777.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4409-
dc.description.abstractNumerous studies have attempted to restore the function of the tumour suppressor p53 as an anticancer strategy through gene delivery. However, most studies employed non-bacterial vectors to deliver p53. Various facultative and obligate anaerobic bacteria have been proposed as vectors because of their intrinsic tumour targeting ability and antitumour activity. Salmonella enterica Typhimurium is the most studied bacterial vector in anticancer therapy. We used the previously designed χ11218 strain of S. enterica Typhimurium, displaying regulated delayed lysis, as a vector for delivering p53 to human bladder carcinoma cells, restoring wild-type p53 protein function. We cloned p53 into pYA4545 (containing a eukaryotic expression system) to generate the χ11218 pYA4545p53 strain. Cloning of p53 did not affect the growth or interfere with the invasive and replicative capacity of χ11218 bacteria in tumour cells. Human bladder carcinoma cells (expressing mutated p53) transfected with pYA4545p53 showed a significant increase in the expression of p53 protein. We demonstrated that p53 supplied by χ11218 significantly decreased the viability of human bladder cancer cells in a dose-dependent manner. This study demonstrates the applicability of the attenuated χ11218 strain as a vector for DNA plasmids expressing tumour suppressor genes.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(COLCIENCIAS) Departamento Administrativo de Ciencia, Tecnología e Innovaciónpt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofLetters in Applied Microbiologypt_BR
dc.rightsRestricted accesspt_BR
dc.titlep53 gene delivery via a recombinant Salmonella enterica Typhimurium leads to human bladder carcinoma cell death in vitropt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1111/lam.13777pt_BR
dc.identifier.urlhttps://doi.org/10.1111/lam.13777pt_BR
dc.contributor.external(UNICAMP) Universidade Estadual de Campinaspt_BR
dc.subject.keywordtumour suppressorpt_BR
dc.subject.keywordSalmonella enterica Typhimuriumpt_BR
dc.subject.keywordgene therapypt_BR
dc.subject.keywordbladder carcinomapt_BR
dc.relation.ispartofabbreviatedLett Appl Microbiolpt_BR
dc.identifier.citationabntin press, jun. 2022pt_BR
dc.identifier.citationvancouver2022 June;in presspt_BR
dc.contributor.butantanMódolo, Diego Grando|:Técnico|:(LG) Lab. Genéticapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/10051-2pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/06134-4pt_BR
dc.sponsorship.butantan(COLCIENCIAS) Departamento Administrativo de Ciencia, Tecnología e Innovación¦¦2016/77pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦88887.595532/2020-00pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦309380/2019-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
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item.openairetypeArticle-
item.languageiso639-1English-
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