Presence of adhesin related genes in enteroaggregative Escherichia coli strains causing diarrhea
Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor | Lab. Bacteriologia | pt_BR |
| dc.date.accessioned | 2022-07-18T11:56:21Z | - |
| dc.date.available | 2022-07-18T11:56:21Z | - |
| dc.date.issued | 2022 | pt_BR |
| dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/4425 | - |
| dc.description.sponsorship | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo | pt_BR |
| dc.format | pt_BR | |
| dc.language.iso | English | pt_BR |
| dc.rights | Open access | pt_BR |
| dc.title | Presence of adhesin related genes in enteroaggregative Escherichia coli strains causing diarrhea | pt_BR |
| dc.title.alternative | Presença de genes relacionados com adesinas em amostras de Escherichia coli enteroagregativa causadoras de diarreia | pt_BR |
| dc.type | Dataset | pt_BR |
| dc.subject.keyword | enteroaggregative Escherichia coli | pt_BR |
| dc.subject.keyword | aggregative-adherence fimbriae | pt_BR |
| dc.subject.keyword | aggregative-forming pilus | pt_BR |
| dc.subject.keyword | CS22 | pt_BR |
| dc.subject.keyword | adhesins | pt_BR |
| dc.subject.keyword | AAF | pt_BR |
| dc.subject.keyword | AFP | pt_BR |
| dc.contributor.butantan | Abe, Cecilia Mari|:Pesquisador|:Lab. Bacteriologia | pt_BR |
| dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/04144-0 | pt_BR |
| dc.identifier.bvscc | BR78.1 | pt_BR |
| dc.identifier.bvsdb | IBProd | pt_BR |
| dc.contributor.researcher | Abe, Cecilia Mari | pt_BR |
| dc.date.modified | 2022-07-13 | - |
| dc.description.dbindexed | Yes | pt_BR |
| dc.description.file | PCR screening for AAF I-IV (.pdf): Multiplex PCR was used to detect the genes encoding the major pilin subunits of AAF/I to IV (aggA, aafA, agg3A and agg4A, respectively), and agg3/4C, corresponding to the usher of AAF/III, IV and V in 110 aatA+ EAEC strains. PCR screening for AAF V (.pdf): Simplex PCR was used to detect the presence of agg5A, encoding the AAF/V major pilin subunit in 110 aatA+ EAEC strains. PCR screening for AFP (.pdf): Simplex PCR was used to detect the presence of afpA1 and afpR, which are part of the AFP-encoding operon in 110 aatA+ EAEC strains. PCR screening for aggR (.pdf): Simplex PCR was used to detect the presence of aggR, encoding the master EAEC virulence regulator in 110 aatA+ EAEC strains. PCR screening for cseA (.pdf): Simplex PCR was used to detect the presence of cseA, encoding the ETEC colonization factor CS22 in 110 aatA+ EAEC strains. List of aatA+ EAEC strains and compiled data (.pdf): All the 110 aatA+ strains were submitted to adherence assays to determine their adherence pattern on HEp-2 cells. They were also submitted to polymerase chain reactions (PCR) analysis to detect the following virulence genes: aggA, aafA, agg3A and agg4A (genes respectively encoding the major pilin subunits of AAF/I to IV); agg3/4C (usher of AAF/III, IV and V); agg5A (encoding the AAF/V major pilin subunit); aggR (encoding the master EAEC virulence regulator); afpA1 and afpR (part of the AFP-encoding operon); and cseA (encoding the ETEC colonization factor CS22). | pt_BR |
| item.languageiso639-1 | English | - |
| item.grantfulltext | open | - |
| item.fulltext | Com Texto completo | - |
| item.openairetype | Dataset | - |
| Appears in Collections: | Dados de Pesquisa (Dataset) | |
Files in This Item:
List of aatA+ EAEC strains and compiled data.pdf
Description: All the 110 aatA+ strains were submitted to adherence assays to determine their adherence pattern on HEp-2 cells. They were also submitted to polymerase chain reactions (PCR) analysis to detect the following virulence genes: aggA, aafA, agg3A and agg4A (genes respectively encoding the major pilin subunits of AAF/I to IV); agg3/4C (usher of AAF/III, IV and V); agg5A (encoding the AAF/V major pilin subunit); aggR (encoding the master EAEC virulence regulator); afpA1 and afpR (part of the AFP-encoding operon); and cseA (encoding the ETEC colonization factor CS22).
Size: 383.29 kB
Format: Adobe PDF
View/Open
Description: All the 110 aatA+ strains were submitted to adherence assays to determine their adherence pattern on HEp-2 cells. They were also submitted to polymerase chain reactions (PCR) analysis to detect the following virulence genes: aggA, aafA, agg3A and agg4A (genes respectively encoding the major pilin subunits of AAF/I to IV); agg3/4C (usher of AAF/III, IV and V); agg5A (encoding the AAF/V major pilin subunit); aggR (encoding the master EAEC virulence regulator); afpA1 and afpR (part of the AFP-encoding operon); and cseA (encoding the ETEC colonization factor CS22).
Size: 383.29 kB
Format: Adobe PDF
View/Open
PCR screening for AAF V.pdf
Description: Simplex PCR was used to detect the presence of agg5A, encoding the AAF/V major pilin subunit in 110 aatA+ EAEC strains.
Size: 353.22 kB
Format: Adobe PDF
View/Open
Description: Simplex PCR was used to detect the presence of agg5A, encoding the AAF/V major pilin subunit in 110 aatA+ EAEC strains.
Size: 353.22 kB
Format: Adobe PDF
View/Open
PCR screening for AFP.pdf
Description: Simplex PCR was used to detect the presence of afpA1 and afpR, which are part of the AFP-encoding operon in 110 aatA+ EAEC strains.
Size: 381.13 kB
Format: Adobe PDF
View/Open
Description: Simplex PCR was used to detect the presence of afpA1 and afpR, which are part of the AFP-encoding operon in 110 aatA+ EAEC strains.
Size: 381.13 kB
Format: Adobe PDF
View/Open
PCR screening for aggR.pdf
Description: Simplex PCR was used to detect the presence of aggR, encoding the master EAEC virulence regulator in 110 aatA+ EAEC strains.
Size: 584.86 kB
Format: Adobe PDF
View/Open
Description: Simplex PCR was used to detect the presence of aggR, encoding the master EAEC virulence regulator in 110 aatA+ EAEC strains.
Size: 584.86 kB
Format: Adobe PDF
View/Open
PCR screening for cseA.pdf
Description: Simplex PCR was used to detect the presence of cseA, encoding the ETEC colonization factor CS22 in 110 aatA+ EAEC strains.
Size: 462.81 kB
Format: Adobe PDF
View/Open
Description: Simplex PCR was used to detect the presence of cseA, encoding the ETEC colonization factor CS22 in 110 aatA+ EAEC strains.
Size: 462.81 kB
Format: Adobe PDF
View/Open
PCR screening for AAF I-IV.pdf
Description: Multiplex PCR was used to detect the genes encoding the major pilin subunits of AAF/I to IV (aggA, aafA, agg3A and agg4A, respectively), and agg3/4C, corresponding to the usher of AAF/III, IV and V in 110 aatA+ EAEC strains.
Size: 590.59 kB
Format: Adobe PDF
View/Open
Description: Multiplex PCR was used to detect the genes encoding the major pilin subunits of AAF/I to IV (aggA, aafA, agg3A and agg4A, respectively), and agg3/4C, corresponding to the usher of AAF/III, IV and V in 110 aatA+ EAEC strains.
Size: 590.59 kB
Format: Adobe PDF
View/Open
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