Instability and depolymerization of the exopolysaccharide produced by Haemophilus Influenzae type B during fermentation

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dc.contributor(LDP) Lab. Desenvolvimento de Processospt_BR
dc.contributor.authorCintra, Felipe de Oliveirapt_BR
dc.contributor.authorTakagi, Mickiept_BR
dc.date.accessioned2022-07-21T16:44:02Z-
dc.date.available2022-07-21T16:44:02Z-
dc.date.issued2020pt_BR
dc.identifier.citationCintra FO, Takagi M. Instability and depolymerization of the exopolysaccharide produced by Haemophilus Influenzae type B during fermentation. SL Vaccin Vaccinat J. 2020 Jun; 3(1):117pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4432-
dc.description.abstractHaemophilus influenzae type b (Hib) is a pathogenic bacterium and the major cause of sequalae and deaths among infants due to meningitis. Hib vaccine is constituted of the exopolysaccharide from the cell capsule and conjugated to a carrier protein. The production of the polysaccharide is complicated due to low yields of production and product recovery in the downstream. In this work, the profiles of molecular mass throughout fermentation were investigated. The molecular mass decreased along fermentation time, despite the increase in concentration. The mechanisms of hydrolysis were investigated, with three possibilities considered: alkaline hydrolysis caused by the solution used to maintain pH of the fermentation; presence of hydrolytic activity from the fermentation metabolites and spontaneous hydrolysisat the temperature and pH was evaluated in the conditions of the fermentation and with purified polysaccharide. The results have suggested that there is not significant influence of the strength of the alkali solution used for pH control neither the presence of hydrolases in the supernatant; however spontaneous hydrolysis was verified in a temperature dependent manner and confirmed with purified polysaccharide.pt_BR
dc.description.sponsorship(BNDES) Banco Nacional de Desenvolvimento Econômico e Socialpt_BR
dc.format.extent117pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofSL Vaccines And Vaccination Journalpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleInstability and depolymerization of the exopolysaccharide produced by Haemophilus Influenzae type B during fermentationpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.urlhttps://www.scientificliterature.org/Vaccines/Vaccines-20-117.pdfpt_BR
dc.identifier.citationvolume3pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordPolysaccharidept_BR
dc.subject.keywordHaemophilus influenzae bpt_BR
dc.subject.keywordInstabilitypt_BR
dc.subject.keyworddepolymerizationpt_BR
dc.subject.keywordmolecular weightpt_BR
dc.relation.ispartofabbreviatedSL Vaccin Vaccinat Jpt_BR
dc.identifier.citationabntv. 3, n. 1, 117, jun. 2020pt_BR
dc.identifier.citationvancouver2020 Jun; 3(1):117pt_BR
dc.contributor.butantanCintra, Felipe de Oliveira|:Aluno|:(LDP) Lab. Desenvolvimento de Processos|:PrimeiroAutorpt_BR
dc.contributor.butantanTakagi, Mickie|:Pesquisador|:(LDP) Lab. Desenvolvimento de Processos|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(BNDES) Banco Nacional de Desenvolvimento Econômico e Social¦¦11.2.0322.1/2012pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.openairetypeArticle-
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crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-0978-4070-
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