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Immune response induced in mice by a hybrid rPotD-PdT pneumococcal protein

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dc.contributor(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributorPrograma de Pós-Doutoradopt_BR
dc.contributor.authorConverso, Thiago Rojaspt_BR
dc.contributor.authorGoulart, Cibellypt_BR
dc.contributor.authorRodríguez, Duniapt_BR
dc.contributor.authorGuerra, Maria Eduarda Souzapt_BR
dc.contributor.authorLeite, Luciana Cezar de Cerqueirapt_BR
dc.date.accessioned2022-08-23T13:04:13Z-
dc.date.available2022-08-23T13:04:13Z-
dc.date.issued2022pt_BR
dc.identifier.citationConverso TR, Goulart C, Rodríguez D, Guerra MES, Leite LCC. Immune response induced in mice by a hybrid rPotD-PdT pneumococcal protein. PloS One. 2022 Aug; 17(8): e0273017. doi:10.1371/journal.pone.0273017.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4486-
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extente0273017pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleImmune response induced in mice by a hybrid rPotD-PdT pneumococcal proteinpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1371/journal.pone.0273017pt_BR
dc.contributor.external(USF) Universidade São Franciscopt_BR
dc.identifier.citationvolume17pt_BR
dc.identifier.citationissue8pt_BR
dc.relation.ispartofabbreviatedPloS Onept_BR
dc.identifier.citationabntv. 17, n. 8, e0273017, ago. 2022pt_BR
dc.identifier.citationvancouver2022 Aug; 17(8): e0273017pt_BR
dc.contributor.butantanRodríguez, Dunia|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanLeite, Luciana Cezar de Cerqueira|:Pesquisador|:(LDV) Lab. de Desenvolvimento de Vacinasapt_BR
dc.contributor.butantanGoulart, Cibelly|:Egresso Pós-Doc|:(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2012/04286-3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/24832-6pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/23566-6pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.abstractptStreptococcus pneumoniae is a human pathogen that colonizes the naso and/or oropharynx and can cause otitis, pneumonia, bacteremia and meningitis. To broaden the protection against pneumococcus, several pneumococcal proteins have been investigated as vaccine candidates. In this study we analyzed the immunological response induced by mouse subcutaneous immunization with a fusion of the Polyamine transport protein D (PotD) and a pneumolysin derivative (PdT), resulting in a hybrid rPotD-PdT protein. Immunization of mice with rPotD-PdT induced increased production of nitric oxide, indicating a higher innate immune response. In agreement, immunization of mice with the hybrid protein was more immunogenic than the individual proteins or their combination, eliciting higher antibody levels. The anti-rPotD-PdT IgG displayed increased binding onto the pneumococcal surface. Furthermore, the anti-rPotD-PdT antisera promoted superior opsonophagocytosis as compared with the other tested formulations. However, despite that the encouraging results in vitro, immunization with the hybrid was not sufficient to induce protection against sepsis with a highly virulent pneumococcal strain. taken together, the results suggest that hybrid proteins are an interesting strategy, able to promote improved immune responses, but the inclusion of other antigens may be necessary to promote protection against invasive infections caused by this bacterium.pt_BR
dc.description.dbindexedYespt_BR
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item.openairetypeArticle-
item.fulltextCom Texto completo-
item.languageiso639-1English-
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