Processo para obtenção de um anticorpo marcado, método para diagnóstico de leptospirose in vitro, uso, e, kit para detecção in vitro da presença de cepas de leptospira em fluidos biológicos

Abstract

The present invention is in the field of immunology. The present invention relates to a process for obtaining a labeled antibody which comprises the steps of inoculating a culture of Leptospira interrogans into a rabbit; extraction of blood from rabbits inoculated with the culture of Leptospira interrogans; purification of anti-leptospira interrogans IgG antibody by caprylic acid precipitation; dialysis of the purified antibody; and conjugating the purified antibody to the N-Hydroxysuccinamide dimethyl acridine ester (DMAE-NHS). The present invention further relates to a method for diagnosing leptospirosis in vitro, which consists of contacting biological fluids suspected of containing leptospires with the labeled anti-leptospira interrogans IgG antibody as obtained by the aforementioned process and detecting the binding of the labeled antibody to leptospira. by chemiluminescence. The present invention further relates to the use of labeled antibody, as obtained by the aforementioned process, for the detection of leptospira in vitro and to a kit for in vitro detection of the presence of leptospira strains in biological fluids comprising the anti-leptospira IgG antibody. interrogans associated with the acridine ester DMAE-NHS.