ZIKV-envelope proteins induce specific humoral and cellular immunity in distinct mice strains

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dc.contributor(LABV) Lab. Biotecnologia Viralpt_BR
dc.contributor(LMP) Lab. Multipropósitopt_BR
dc.contributor.authorLunardelli, Victória Alves Santospt_BR
dc.contributor.authorApostolico, Juliana de Souzapt_BR
dc.contributor.authorSouza, Higo Fernando Santospt_BR
dc.contributor.authorCoirada, Fernanda Carolinept_BR
dc.contributor.authorMartinho, Jéssica Amaralpt_BR
dc.contributor.authorAstray, Renato Mancinipt_BR
dc.contributor.authorBoscardin, Silvia Beatrizpt_BR
dc.contributor.authorRosa, Daniela Santoropt_BR
dc.date.accessioned2022-10-04T13:28:20Z-
dc.date.available2022-10-04T13:28:20Z-
dc.date.issued2022pt_BR
dc.identifier.citationLunardelli VAS, Apostolico JS, Souza HFS, Coirada FC, Martinho JA, Astray RM, et al. ZIKV-envelope proteins induce specific humoral and cellular immunity in distinct mice strains. Sci Rep. 2022 Sep; 12: 15733. doi:10.1038/s41598-022-20183-x.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4532-
dc.description.abstractRecent outbreaks of Zika virus (ZIKV) infection have highlighted the need for a better understanding of ZIKV-specific immune responses. The ZIKV envelope glycoprotein (EZIKV) is the most abundant protein on the virus surface and it is the main target of the protective immune response. EZIKV protein contains the central domain (EDI), a dimerization domain containing the fusion peptide (EDII), and a domain that binds to the cell surface receptor (EDIII). In this study, we performed a systematic comparison of the specific immune response induced by different EZIKV recombinant proteins (EZIKV, EDI/IIZIKV or EDIIIZIKV) in two mice strains. Immunization induced high titers of E-specific antibodies which recognized ZIKV-infected cells and neutralized the virus. Furthermore, immunization with EZIKV, EDI/IIZIKV and EDIIIZIKV proteins induced specific IFNγ-producing cells and polyfunctional CD4+ and CD8+ T cells. Finally, we identified 4 peptides present in the envelope protein (E1–20, E51–70, E351–370 and E361–380), capable of inducing a cellular immune response to the H-2Kd and H-2Kb haplotypes. In summary, our work provides a detailed assessment of the immune responses induced after immunization with different regions of the ZIKV envelope protein.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent15733pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleZIKV-envelope proteins induce specific humoral and cellular immunity in distinct mice strainspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1038/s41598-022-20183-xpt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume12pt_BR
dc.relation.ispartofabbreviatedSci Reppt_BR
dc.identifier.citationabntv. 12, 15733, set. 2022pt_BR
dc.identifier.citationvancouver2022 Sep; 12: 15733pt_BR
dc.contributor.butantanAstray, Renato Mancini|:Pesquisador|:(LABV) Lab. Biotecnologia Viral|:(LMP) Lab. Multipropósitopt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/17471-7pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2021/13004-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/05320-7pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦465434/2014-2pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.grantfulltextembargo_20990101-
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