The Aedes aegypti peritrophic matrix controls arbovirus vector competence through HPx1, a heme–induced peroxidase

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dc.contributorLab. Expressão Gênica em Eucariotospt_BR
dc.contributor(LCC) Lab. Ciclo Celularpt_BR
dc.contributor.authorTalyuli, Octavio A. C.pt_BR
dc.contributor.authorOliveira, Jose Henrique M.pt_BR
dc.contributor.authorBottino-Rojas, Vanessapt_BR
dc.contributor.authorSilveira, Gilbert de Oliveirapt_BR
dc.contributor.authorAlvarenga, Patricia H.pt_BR
dc.contributor.authorBarletta, Ana Beatriz F.pt_BR
dc.contributor.authorKantor, Asher M.pt_BR
dc.contributor.authorPaiva-Silva, Gabriela O.pt_BR
dc.contributor.authorBarillas-Mury, Carolinapt_BR
dc.contributor.authorOliveira, Pedro L.pt_BR
dc.identifier.citationTalyuli OA.C., Oliveira JHM., Bottino-Rojas V, Silveira GO, Alvarenga PH., Barletta ABF., et al. The Aedes aegypti peritrophic matrix controls arbovirus vector competence through HPx1, a heme–induced peroxidase. PLoS Pathog. 2023 Feb; 19(2)e1011149. doi:10.1371/journal.ppat.1011149.pt_BR
dc.description.abstractAedes aegypti mosquitoes are the main vectors of arboviruses. The peritrophic matrix (PM) is an extracellular layer that surrounds the blood bolus. It acts as an immune barrier that prevents direct contact of bacteria with midgut epithelial cells during blood digestion. Here, we describe a heme-dependent peroxidase, hereafter referred to as heme peroxidase 1 (HPx1). HPx1 promotes PM assembly and antioxidant ability, modulating vector competence. Mechanistically, the heme presence in a blood meal induces HPx1 transcriptional activation mediated by the E75 transcription factor. HPx1 knockdown increases midgut reactive oxygen species (ROS) production by the DUOX NADPH oxidase. Elevated ROS levels reduce microbiota growth while enhancing epithelial mitosis, a response to tissue damage. However, simultaneous HPx1 and DUOX silencing was not able to rescue bacterial population growth, as explained by increased expression of antimicrobial peptides (AMPs), which occurred only after double knockdown. This result revealed hierarchical activation of ROS and AMPs to control microbiota. HPx1 knockdown produced a 100-fold decrease in Zika and dengue 2 midgut infection, demonstrating the essential role of the mosquito PM in the modulation of arbovirus vector competence. Our data show that the PM connects blood digestion to midgut immunological sensing of the microbiota and viral infections.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FINEP) Financiadora de Estudos e Projetospt_BR
dc.description.sponsorship(FAPERJ) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiropt_BR
dc.description.sponsorship(NIH) National Institutes of Healthpt_BR
dc.relation.ispartofPLoS Pathogenspt_BR
dc.rightsOpen accesspt_BR
dc.titleThe Aedes aegypti peritrophic matrix controls arbovirus vector competence through HPx1, a heme–induced peroxidasept_BR
dc.contributor.external(UFRJ) Universidade Federal do Rio de Janeiropt_BR
dc.contributor.external(UFSC) Universidade Federal de Santa Catarinapt_BR
dc.contributor.external(UC) University of Californiapt_BR
dc.contributor.external(NIAID) National Institute of Allergy and Infectious Diseasespt_BR
dc.contributor.external(INCT - EM) Instituto Nacional de Ciência e Tecnologia em Entomologia Molecularpt_BR
dc.relation.ispartofabbreviatedPLoS Pathogpt_BR
dc.identifier.citationabntv. 19, n. 2, e1011149, fev. 2023pt_BR
dc.identifier.citationvancouver2023 Feb; 19(2)e1011149pt_BR
dc.contributor.butantanSilveira, Gilbert de Oliveira|:Aluno|:Lab. Expressão Gênica em Eucariotos|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantan(FINEP) Financiadora de Estudos e Projetos¦¦pt_BR
dc.sponsorship.butantan(FAPERJ) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro¦¦pt_BR
dc.sponsorship.butantan(NIH) National Institutes of Health¦¦Z01AI000947pt_BR
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