Staphylococcus aureus-cure-associated antigens Elicit type 3 immune memory T cells

Butantan affiliation
Publication type
Access rights
Open access
Terms of use
Appears in Collections:
Background: Staphylococcus aureus is one of the most frequently major mastitis pathogens that cause clinical and subclinical mastitis worldwide. Current antimicrobial treatments are usually ineffective, and the commercially available vaccines lack proven effectiveness. The immunological response elicited by the recombinant S. aureus-cure-associated proteins phosphoglycerate kinase (PGK), enolase (ENO), and elongation factor-G (EF-G) in combination with the granulocyte-macrophage colony-stimulating factor (GM-CSF) DNA vaccination was studied in this work. Methods: Here, twenty-three C57BL/6 mice were divided into four groups and vaccinated with: G1: none (control); G2: GM-CSF DNA plasmid DNA vaccine; G3: the combination of EF-G+ENO+PGK; and G4: the combinations of EF-G+ENO+PGK proteins plus GM-CSF plasmid DNA vaccine. After 44 days, spleen cells were collected for immunophenotyping and lymphocyte proliferation evaluation by flow cytometry upon S. aureus stimulus. Results: Immunization with the three S. aureus recombinant proteins alone resulted in a higher percentage of IL-17A+ cells among CD8+ T central memory cells, as well as the highest intensity of IL-17A production by overall lymphocytes indicating that the contribution of the combined lymphocyte populations is crucial to sustaining a type 3 cell immunity environment. Conclusion: The immunization with three S. aureus-cure-associated recombinant proteins triggered type 3 immunity, which is a highly interesting path to pursue an effective bovine S. aureus mastitis vaccine.
Santos KR., Souza FN., Ramos-Sanchez EM., Fotoran WL. Staphylococcus aureus-cure-associated antigens Elicit type 3 immune memory T cells. Antibiotics. 2022 Dec; 11(12)1831. doi:10.3390/antibiotics11121831.
Link to cite this reference
Journal title
Issue Date

Files in This Item:

Size: 1.95 MB
Format: Adobe PDF
Show full item record

This item is licensed under a Creative Commons License Creative Commons