Use of nanostructured silica SBA-15 as an oral vaccine adjuvant to control mycoplasma hyopneumoniae in swine production

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dc.contributor(LBI) Lab. Imunoquímicapt_BR
dc.contributor.authorStorino, Gabriel Y.pt_BR
dc.contributor.authorPetri, Fernando A. M.pt_BR
dc.contributor.authorMechler-Dreibi, Marina L.pt_BR
dc.contributor.authorSant'anna, Osvaldo Augustopt_BR
dc.date.accessioned2023-04-17T12:07:59Z-
dc.date.available2023-04-17T12:07:59Z-
dc.date.issued2023pt_BR
dc.identifier.citationStorino GY., Petri FA.M., Mechler-Dreibi ML., Sant'anna OA. Use of nanostructured silica SBA-15 as an oral vaccine adjuvant to control mycoplasma hyopneumoniae in swine production. Int J Mol Sci. 2023 Apr; 24(7):6591. doi:10.3390/ijms24076591.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/4859-
dc.description.abstractMycoplasma hyopneumoniae is a difficult-to-control bacterium since commercial vaccines do not prevent colonization and excretion. The present study aimed to evaluate the performance of an orally administered vaccine composed of antigens extracted from Mycoplasma hyopneumoniae and incorporated into mesoporous silica (SBA-15), which has an adjuvant-carrier function, aiming to potentiate the action of the commercial intramuscular vaccine. A total of 60 piglets were divided into four groups (n = 15) submitted to different vaccination protocols as follows, Group 1: oral SBA15 + commercial vaccine at 24 days after weaning, G2: oral vaccine on the third day of life + vaccine commercial vaccine at 24 days, G3: commercial vaccine at 24 days, and G4: commercial vaccine + oral vaccine at 24 days. On the first day, the piglets were weighed and, from the third day onwards, submitted to blood collections for the detection and quantification of anti-Mycoplasma hyopneumoniae IgG. Nasal swabs were collected to monitor IgA by ELISA, and oropharyngeal swabs were used to assess the bacterial load by qPCR. Biological samples were collected periodically from the third day of life until the 73rd day. At 41 days of life, 15 individuals of the same age, experimentally challenged with an inoculum containing M. hyopneumoniae, were co-housed with the animals from groups (1 to 4) in a single pen to increase the infection pressure during the nursery period. At 73 days, all piglets were euthanized, and lungs were evaluated by collecting samples for estimation of bacterial load by qPCR. Quantitative data obtained from physical parameters and laboratory investigation were analyzed by performing parametric or non-parametric statistical tests. Results indicate that animals from G2 showed smaller affected lung areas compared to G3. Animals from G2 and G4 had a low prevalence of animals shedding M. hyopneumoniae at 61 days of age. Additionally, no correlation was observed between lung lesions and M. hyopneumoniae load in lung and BALF samples in animals that received the oral vaccine, while a strong correlation was observed in other groups. In the present study, evidence points to the effectiveness of the oral vaccine developed for controlling M. hyopneumoniae in pig production under field conditions.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent6591pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofInternational Journal of Molecular Sciencespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleUse of nanostructured silica SBA-15 as an oral vaccine adjuvant to control mycoplasma hyopneumoniae in swine productionpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/ijms24076591pt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.contributor.external(UFV) Universidade Federal de Viçosapt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume24pt_BR
dc.identifier.citationissue7pt_BR
dc.subject.keywordanimal healthpt_BR
dc.subject.keywordimmunologypt_BR
dc.subject.keywordinfectious diseasespt_BR
dc.subject.keywordrespiratory diseasespt_BR
dc.subject.keywordvaccinologypt_BR
dc.relation.ispartofabbreviatedInt J Mol Scipt_BR
dc.identifier.citationabntv. 24, n. 7, 6591, abr. 2023pt_BR
dc.identifier.citationvancouver2023 Apr; 24(7):6591pt_BR
dc.contributor.butantanSant'anna, Osvaldo Augusto|:Pesquisador|:(LBI) Lab. Imunoquímica|:pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2021/11914- 0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/17844-8pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/08582-5pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/07007-7pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2020/05198-7pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦140495/2019-3pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦316447/2021-8pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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