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Recombinant human TIM-3 ectodomain expression and characterization.

Translated title
Expressão e caracterização do ectodomínio recombinante do TIM-3 humano
Responsible researcher
Luz, Daniela 
Butantan affiliation
Lab. Bacteriologia 
External affiliation
(IPEN) Instituto de Pesquisas Energéticas e Nucleares ;  (USP) Universidade de São Paulo ;  A. C. Camargo Cancer Center ;  Instituto D'or de Pesquisa e Ensino 
Publication type
Dataset
Language
English
File format
.doc, .png, .tiff, .xls
Updated on
22-May-2023
Access rights
Open access
Appears in Collections:
Dataset description
DNA sequences (.doc): SDS-Page and immunoblotting (.png files): Immunofluorescence - A549_HEK293 TIM-3-ECD 161222 (.tiff files): PBMC activation assay data (.xls): TIM-3 Circular dichroism (.xls): TIM-3 ELISA (.xls)
Reference
Luz, D. Recombinant human TIM-3 ectodomain expression and characterization. [dataset]. Instituto Butantan Repository. 2023.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/4918
Keywords
TIM-3;  inclusion bodies;  bacteria expression;  active recombinant protein;  membrane protein
Funding agency
(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo 
FAPESP grants
2022/04560-0;  2021/04307-0
Issue Date
2023

Files in This Item:

DNA Sequences.docx
Description: Cloning DNA sequences
Size: 225.35 kB
Format: Microsoft Word XML
View/Open
Immunoblotting TIM-3-ECD expression and purification.png
Description:
Size: 3.13 MB
Format: image/png
Thumbnail
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PBMC activation assay data.xlsx
Description:
Size: 8.15 kB
Format: Microsoft Excel XML
View/Open
SDS-PAGE TIM-3 ECD expression and purification.jpeg
Description:
Size: 96.16 kB
Format: JPEG
Thumbnail
View/Open
TIM-3 Circular dichroism.xlsx
Description:
Size: 30.59 kB
Format: Microsoft Excel XML
View/Open
TIM-3 ELISA.xlsx
Description:
Size: 9.45 kB
Format: Microsoft Excel XML
View/Open
A549 conjugate control.tif
Description: Immunofluorescence - A549_HEK293 TIM-3-ECD 161222
Size: 4.8 MB
Format: TIFF
View/Open
A549 control.tif
Description: Immunofluorescence - A549_HEK293 TIM-3-ECD 161222
Size: 4.8 MB
Format: TIFF
View/Open
A549_TIM-3 1h treatment.tif
Description: Immunofluorescence - A549_HEK293 TIM-3-ECD 161222
Size: 3.6 MB
Format: TIFF
View/Open
HEK293 conjugate control.tif
Description: Immunofluorescence - A549_HEK293 TIM-3-ECD 161222
Size: 4.8 MB
Format: TIFF
View/Open
HEK293 control.tif
Description: Immunofluorescence - A549_HEK293 TIM-3-ECD 161222
Size: 4.8 MB
Format: TIFF
View/Open
HEK293_TIM-3 1h treatment.tif
Description: Immunofluorescence - A549_HEK293 TIM-3-ECD 161222
Size: 4.8 MB
Format: TIFF
View/Open
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