Identification of miRnas with possible prognostic roles for HAM/TSP

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dc.contributorLab. Imunopatologiapt_BR
dc.contributor.authorde Souza, Daniela Raguer Valadãopt_BR
dc.contributor.authorPessôa, Rodrigopt_BR
dc.contributor.authorNukui, Youkopt_BR
dc.contributor.authorClissa, Patricia Biancapt_BR
dc.identifier.citationde Souza DRV, Pessôa R, Nukui Y, Clissa PB. Identification of miRnas with possible prognostic roles for HAM/TSP. Virulence. 2023 Jul; 14(1):2230015. doi:10.1080/21505594.2023.2230015.pt_BR
dc.description.abstractHuman T-cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropic spastic paraparesis (HAM/TSP) is an insidiously progressive spinal cord disease for which there is no effective treatment. There is great interest in developing potential biomarkers to predict the pathogenesis of HAM/TSP disease. In this study, Illumina Massive Parallel Sequencing (MPS) technology was used to investigate the cellular global noncoding RNAome expression profile in HAM/TSP patients (n = 10), asymptomatic HTLV-1-infected carriers (ASP, n = 8), and a second group of healthy controls (n = 5). Various bioinformatics tools were used to align, annotate, and profile the sRNA-MPS reads. Among the 402 sRNAs detected, 251 were known and 50 were potentially novel sRNAs in the HAM and ASP groups compared with the HC group. Sixty-eight known sRNAs were significantly different between the ASP and HAM groups. Eighty-eight mature miRNAs were downregulated in subjects from HAM compared with ASP. Three of these miRs (hsa-miR-185-5p, 32-5p, and 192-5p) have the potential to be used as biomarkers for predicting the pathogenesis of HAM/TSP. The seven most deregulated miRs target genes have been associated with a variety of biological processes and molecular functions. The reactome pathways relevant to our findings provide a rich source of data and offer the opportunity to better understand sRNA regulation and function in HTLV-1 pathophysiology. To the best of our knowledge, this study is the first to demonstrate evaluates sRNAs in HTLV-1 patients with HAM/TSP.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.rightsOpen accesspt_BR
dc.titleIdentification of miRnas with possible prognostic roles for HAM/TSPpt_BR
dc.rights.licenseCC BY-NCpt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.externalInstituto de Infectologia Emílio Ribaspt_BR
dc.subject.keywordsmall RNApt_BR
dc.subject.keywordHAM/ TSPpt_BR
dc.subject.keywordmassive parallel sequencingpt_BR
dc.identifier.citationabntv. 14, n. 1, 2230015, jul. 2023pt_BR
dc.identifier.citationvancouver2023 Jul; 14(1):2230015pt_BR
dc.contributor.butantanClissa, Patricia Bianca|:Pesquisador|:Lab. Imunopatologia|:pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2022/09354-9pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2011/ 12297-2pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/24596-2pt_BR
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