Análise da urina dos camundongos ofertados com água filtrada e autoclavada (esterilizada)

Abstract

Water is considered one of the most important concerns in the world in terms of its use and maintenance as a universal good. Drinking water is considered when it poses no risk to health, is free of chemical and biological agents harmful to human and animal health, and is therefore fit for consumption. The supply of water in vivariums, whether for experimentation or production, may not present a water quality that is satisfactory for consumption, as the supply comes from the ater and sewage treatment system, which may still contain microorganisms and residues, such as iron, chlorine, heavy metals and even other organisms harmful to animal health. The vivariums have physical and chemical systems such as autoclaving, filtration, chlorination and acidification to contain the amount of waste and infectious agents in the water offered to the animals and the laboratory routine -LENA DE SÁ, et al. 2001-. Chemical processes require the need for regular tests to validate the water, ensuring quality for animal ingestion. Filtration, which is a physical method, manages to block undesirable substances through granulation -sand and carbon- and autoclave sterilization becomes more used. -KURTZ, et al. 2020-. In vivariums, whether for experimentation or creation, in addition to filtration, the autoclave becomes one of the indispensable items, as there is a requirement that the animals do not suffer external interference to the point of harming or altering the results of the experiments. NORMATIVA N.33 CONCEA, 2016. The present project aimed to compare water samples, offered to mice, which went through the filtration and autoclaved process, and to determine the parameters of the urine samples. The results of the water analysis were identical between both samples and the values of the reactive strip markers in the urine, showed results within the reference values, demonstrating that filtered water or autoclaved water is effective to maintain the health of the animals.