The dynamic subcellular localisation of Rad1 is cell cycle dependent in Leishmania major
Appears in Collections:
The subcellular localisation of Rad1, a subunit of the Leishmania major 9-1-1 complex, remains unexplored. Herein, we reveal that Rad1 localises predominantly to the nucleus. Upon hydroxyurea treatment, the diffuse nuclear localisation of Rad1 becomes more punctate, suggesting that Rad1 is responsive to replication stress. Moreover, Rad1 localisation correlates with cell cycle progression. In the majority of G1 to early S-phase cells, Rad1 localises predominantly to the nucleus. As cells progress from late-S phase to mitosis, Rad1 relocalizes to both the nucleus and the cytoplasm in ∼90 % of cells. This pattern of distribution is different from Rad9 and Hus1, which remain nuclear throughout the cell cycle, suggesting Leishmania Rad1 may regulate 9-1-1 activities and/or perform relevant functions outside the 9-1-1 complex.
Santos EV., Damasceno JD., Gomez RO, Rosales R, Black JA., McCulloch R, et al. The dynamic subcellular localisation of Rad1 is cell cycle dependent in Leishmania major. Exp Parasitol. 2023 Dec; 255:108639. doi:10.1016/j.exppara.2023.108639.
Link to cite this reference
Show full item record
The access to the publications deposited in this repository respects the licenses from journals and publishers.