Standardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenom

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dc.contributorLab. Bioquímicapt_BR
dc.contributorPrograma de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributor.authorOrsi, Ricardo Oliveirapt_BR
dc.contributor.authorZaluskic, Rodrigopt_BR
dc.contributor.authorBarros, Luciana Curtolo dept_BR
dc.contributor.authorBarraviera, Beneditopt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorJunior, Rui Seabra Ferreirapt_BR
dc.date.accessioned2024-02-19T18:55:14Z-
dc.date.available2024-02-19T18:55:14Z-
dc.date.issued2024pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/5250-
dc.description.abstractAfricanized bees have spread across the Americas since 1956 and consequently resulted in human and animal deaths attributed to massive attacks related to exposure from Argentina to the USA. In Brazil, more than 100,000 accidents were registered in the last 5 years with a total of 303 deaths. To treat such massive attacks, Brazilian researchers developed the first specific antivenom against Africanized honey bee sting exposure. This unique product, the first of its kind in the world, has been safely tested in 20 patients during a Phase 2 clinical trial. To develop the antivenom, a standardized process was undertaken to extract primary venom antigens from the Africanized bees for immunization of serum-producing horses. This process involved extracting, purifying, fractionating, characterizing, and identifying the venom (apitoxin) employing mass spectrometry to generate standardized antigen for hyperimmunization of horses using the major toxins (melittin and its isoforms and phospholipase A2). The current guide describes standardization of the entire production chain of venom antigens in compliance with good manufacturing practices (GMP) required by regulatory agencies. Emphasis is placed upon the welfare of bees and horses during this process, as well as the development of a new biopharmaceutical to ultimately save lives.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent73-90pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Toxicology and Environmental Health - Part Bpt_BR
dc.rightsRestricted accesspt_BR
dc.titleStandardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenompt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1080/10937404.2023.2300786pt_BR
dc.identifier.urlhttps://doi.org/10.1080/10937404.2023.2300786pt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.contributor.external(UFMS) Universidade Federal de Mato Grosso do Sulpt_BR
dc.identifier.citationvolume27pt_BR
dc.identifier.citationissue2pt_BR
dc.subject.keywordAfricanized honeybeept_BR
dc.subject.keywordApis melliferapt_BR
dc.subject.keywordgood manufacturing practicespt_BR
dc.subject.keywordhyperimmunizationpt_BR
dc.subject.keywordstandard operating procedurespt_BR
dc.relation.ispartofabbreviatedJ Toxicol Environ Health B Crit Revpt_BR
dc.identifier.citationabntv. 27, n. 2, p. 73-90, jan. 2024pt_BR
dc.identifier.citationvancouver2024 Jan; 27(2):73:90pt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador|:Lab. Bioquímica|:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦563582/2010-3pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦441463/2019-3pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦23038.006285/2011-21pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦23038.008557/2010pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2021/11936-3pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦301608/2022-9pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦304720/2020-8pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦306339/2020-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.openairetypeArticle-
item.fulltextSem Texto completo-
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