Liposome-based dry powder vaccine immunization targeting the lungs induces broad protection against pneumococcus

Registo de metadados completa
Campo DCValoridioma
dc.contributorLab. Bacteriologiapt_BR
dc.contributor(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorRodrigues, T. C.pt_BR
dc.contributor.authorFigueiredo, D. B.pt_BR
dc.contributor.authorGonçalves, Viviane Maimonipt_BR
dc.contributor.authorKaneko, K.pt_BR
dc.contributor.authorSaleem, I. Y.pt_BR
dc.contributor.authorMiyaji, Eliane Namiept_BR
dc.date.accessioned2024-03-11T18:35:46Z-
dc.date.available2024-03-11T18:35:46Z-
dc.date.issued2024pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/5271-
dc.description.abstractStreptococcus pneumoniae is an important human pathogen. Currently used conjugate vaccines are effective against invasive disease, but protection is restricted to serotypes included in the formulation, leading to serotype replacement. Furthermore, protection against non-invasive disease is reported to be considerably lower. The development of a serotype-independent vaccine is thus important and Pneumococcal surface protein A (PspA) is a promising vaccine candidate. PspA shows some diversity and can be classified in 6 clades and 3 families, with families 1 and 2 being the most frequent in clinical isolates. The ideal vaccine should thus induce protection against the two most common families of PspA. The aim of this work was to develop a liposome-based vaccine containing PspAs from family 1 and 2 and to characterize its immune response. Liposomes (LP) composed of dipalmitoylphosphatidylcholine (DPPC) and 3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol (DC-Chol) with or without α-galactosylceramide (α-GalCer) were produced by microfluidics, encapsulating PspA from clade 1 (PspA1, family 1) and/or clade 4 (PspA4Pro, family 2) followed by spray-drying with trehalose to form nanocomposite microparticles carriers (NCMP). LP/NCMPs showed good stability and preservation of protein activity. LP/NCMPs containing PspA1 and/or PspA4Pro were used for immunization of mice targeting the lungs. High serum IgG antibody titers against both PspA1 and PspA4Pro were detected in animals immunized with LP/NCMPs containing α-GalCer, with a balance of IgG1 and IgG2a titers. IgG in sera from immunized mice bound to pneumococcal strains from different serotypes and expressing different PspA clades, indicating broad recognition. Mucosal IgG and IgA were also detected. Importantly, immunization with LP/NCMPs induced full protection against strains expressing PspAs from family 1 and 2. Furthermore, CD4+ resident memory T cells were detected in the lungs of the immunized animals that survived the challenge.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(MRC) Medical Research Councilpt_BR
dc.description.sponsorshipBactivac Networkpt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent184-198pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Controlled Releasept_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleLiposome-based dry powder vaccine immunization targeting the lungs induces broad protection against pneumococcuspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1016/j.jconrel.2024.02.028pt_BR
dc.contributor.external(LJMU) Liverpool John Moores Universitypt_BR
dc.identifier.citationvolume368pt_BR
dc.subject.keywordStreptococcus pneumoniaept_BR
dc.subject.keywordPneumoniapt_BR
dc.subject.keywordprotein antigenpt_BR
dc.subject.keywordvaccinept_BR
dc.subject.keywordLiposomespt_BR
dc.subject.keywordmucosal immunitypt_BR
dc.relation.ispartofabbreviatedJ Control Releasept_BR
dc.identifier.citationabntv. 368, p. 184-198, abr. 2024pt_BR
dc.identifier.citationvancouver2024 Apr; 368:184-198pt_BR
dc.contributor.butantanGonçalves, Viviane Maimoni|:Pesquisador|:(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanMiyaji, Eliane Namie|:Pesquisador|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/50413-8pt_BR
dc.sponsorship.butantan(MRC) Medical Research Council¦¦MR/P022758/1pt_BR
dc.sponsorship.butantanBactivac Network¦¦BVNCP4-03pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/26090-7pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/10497-6pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦303072/2018-0pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦303049/2022-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.openairetypeArticle-
item.grantfulltextopen-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-0980-8116-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-1689-5366-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Aparece nas Coleções:Artigos


Arquivos neste item

Liposome-based dry powder vaccine immunization target.pdf
Descrição:
Tamanho: 6.29 MB
Formato: Adobe PDF
Ver/Aberto
Mostrar registro simples do item

Este item está licenciada sob uma Licença Creative Commons Creative Commons