Detecção de papilomavírus bovino (BPV) em amostras de caprinos provenientes do estado do Pernambuco

Abstract

Papillomaviruses (PVs) represent a diverse group of viruses, with approximately 55- 60nm in diameter and a genome composed of circular double-stranded DNA, ranging from 7,000bp to 8,600bp. Although most types have been described in humans, PVs have been identified in other hosts, particularly domestic species, birds, and reptiles. Generally, PVs are species-specific, restricted to a host species. However, bovine papillomavirus (BPV) stands out for infecting different species beyond its original host. This study aimed to perform the molecular diagnosis of PVs from skin lesion samples of goats (Capra aegagrus hircus). Lesions from seven goats from the state of Pernambuco were collected. The clinical material underwent DNA extraction, PCR reactions using the FAP 59/64 primer set and agarose gel electrophoresis. The corresponding band was purified and cloned into a bacterial vector using competent cells (E. coli, strain DH5α). After colony selection, recombinant plasmids were extracted, purified, and subjected to digestion to check for the presence of the insert. Subsequently, plasmid samples were sent to the Human Genome Studies Center at the University of São Paulo for DNA sequencing. The generated sequences were compared to a public database (NCBI) for identification. So far, four viral sequences have been identified in DNA samples corresponding to three animals, including two putative new types of BPV, in addition to co-infection with BPV5 and BPV13 types. To the best of our knowledge, this work reports the first description of bovine papillomavirus (BPV) sequences in goats.