EPEC autotransporter adhesin (Eaa): a novel adhesin identified in atypical enteropathogenic Escherichia coli
Registo de metadados completa
| Campo DC | Valor | idioma |
|---|---|---|
| dc.contributor | Lab. Bacteriologia | pt_BR |
| dc.date.accessioned | 2024-12-10T18:34:51Z | - |
| dc.date.available | 2024-12-10T18:34:51Z | - |
| dc.date.issued | 2024 | pt_BR |
| dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/5524 | - |
| dc.description.sponsorship | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo | pt_BR |
| dc.format | pt_BR | |
| dc.language.iso | English | pt_BR |
| dc.rights | Open access | pt_BR |
| dc.title | EPEC autotransporter adhesin (Eaa): a novel adhesin identified in atypical enteropathogenic Escherichia coli | pt_BR |
| dc.title.alternative | EPEC autotransporter adhesin (Eaa): uma nova adesina identificada em Escherichia coli enteropatogênica atípica | pt_BR |
| dc.type | Dataset | pt_BR |
| dc.contributor.external | (UNESP) Universidade Estadual Paulista Júlio de Mesquita Filho | pt_BR |
| dc.contributor.external | (IAL) Instituto Adolfo Lutz | pt_BR |
| dc.contributor.external | (UQ) University of Queensland | pt_BR |
| dc.contributor.external | University of Maryland School of Medicine | pt_BR |
| dc.subject.keyword | EPEC | pt_BR |
| dc.subject.keyword | Adhesin | pt_BR |
| dc.subject.keyword | Autotransporter | pt_BR |
| dc.subject.keyword | Enteropathogenic Escherichia coli | pt_BR |
| dc.contributor.butantan | Waldir Pereira Elias Junior:Autor de correspondência | pt_BR |
| dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/14821-7 | pt_BR |
| dc.identifier.bvscc | BR78.1 | pt_BR |
| dc.subject.researchline | Patogenicidade bacteriana | pt_BR |
| dc.contributor.researcher | Elias, Waldir Pereira | pt_BR |
| dc.description.dbindexed | Yes | pt_BR |
| dc.description.file | Autoaggregation assays under shaking incubation: Optical Density (OD) readings of replicates; Autoaggregation assays under static incubation: Optical Density (OD) readings of replicates; Biofilm assays on polystyrene surface: Optical Density (OD) readings of replicates; Biofilm assays on glass surface: Optical Density (OD) readings of replicates; ELISA assays for binding to extracellular matrix components: Optical Density (OD) readings of replicates. | pt_BR |
| dc.sponsorship.fapesp | 2017/14821-7 | pt_BR |
| item.languageiso639-1 | English | - |
| item.fulltext | Com Texto completo | - |
| item.openairetype | Dataset | - |
| item.grantfulltext | open | - |
| Aparece nas Coleções: | Dados de Pesquisa (Dataset) | |
Arquivos neste item
Autoaggregation_shaking.pdf
Descrição: Autoaggregation assays under shaking incubation at 37°C. All cultures were homogenized before each reading. Missing values represent outliers that were removed or strains that were not tested on a specific experiment. OD readings at 600 nm.
Tamanho: 174.79 kB
Formato: Adobe PDF
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Descrição: Autoaggregation assays under shaking incubation at 37°C. All cultures were homogenized before each reading. Missing values represent outliers that were removed or strains that were not tested on a specific experiment. OD readings at 600 nm.
Tamanho: 174.79 kB
Formato: Adobe PDF
Ver/Aberto
Autoaggregation assays_static conditions.pdf
Descrição: Autoaggregation assays performed under static conditions at 37°C. All cultures were homogenized at the start of experiment and then kept at static conditions for further measurements. The missing values represent an outlier that was removed or strains that were not used on specific experiments. OD readings at 600 nm.
Tamanho: 168.71 kB
Formato: Adobe PDF
Ver/Aberto
Descrição: Autoaggregation assays performed under static conditions at 37°C. All cultures were homogenized at the start of experiment and then kept at static conditions for further measurements. The missing values represent an outlier that was removed or strains that were not used on specific experiments. OD readings at 600 nm.
Tamanho: 168.71 kB
Formato: Adobe PDF
Ver/Aberto
Biofilm assays_polystyrene.pdf
Descrição: Biofilm assays performed on polystyrene surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 126.18 kB
Formato: Adobe PDF
Ver/Aberto
Descrição: Biofilm assays performed on polystyrene surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 126.18 kB
Formato: Adobe PDF
Ver/Aberto
Biofilm assays_glass.pdf
Descrição: Biofilm assays performed on glass surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 129.3 kB
Formato: Adobe PDF
Ver/Aberto
Descrição: Biofilm assays performed on glass surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 129.3 kB
Formato: Adobe PDF
Ver/Aberto
ELISA_Binding to ECM components.pdf
Descrição: ELISA assays to detect binding to extracellular matrix components. Adherence to 1 µg of the extracellular matrix components assay performed using 0.1 µM of the passenger domain (recombinant protein) of Eaa. Readings were taken at 492 nm. Values represent the average of each experiment.
Tamanho: 160.08 kB
Formato: Adobe PDF
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Descrição: ELISA assays to detect binding to extracellular matrix components. Adherence to 1 µg of the extracellular matrix components assay performed using 0.1 µM of the passenger domain (recombinant protein) of Eaa. Readings were taken at 492 nm. Values represent the average of each experiment.
Tamanho: 160.08 kB
Formato: Adobe PDF
Ver/Aberto
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