EPEC autotransporter adhesin (Eaa): a novel adhesin identified in atypical enteropathogenic Escherichia coli
Título traduzido
EPEC autotransporter adhesin (Eaa): uma nova adesina identificada em Escherichia coli enteropatogênica atípica
Pesquisador responsável
Afiliação Butantan
Afiliação externa
Tipo de documento
Dataset
Idioma
English
Formato de arquivo
PDF
Direitos de acesso
Open access
Aparece nas Coleções:
Descrição do conjunto de dados
Autoaggregation assays under shaking incubation: Optical Density (OD) readings of replicates; Autoaggregation assays under static incubation: Optical Density (OD) readings of replicates; Biofilm assays on polystyrene surface: Optical Density (OD) readings of replicates; Biofilm assays on glass surface: Optical Density (OD) readings of replicates; ELISA assays for binding to extracellular matrix components: Optical Density (OD) readings of replicates.
URL permanente para citação desta referência
https://repositorio.butantan.gov.br/handle/butantan/5524
Palavra-chave
Linha de pesquisa
Agência de fomento
Auxílio FAPESP
2017/14821-7
Data de publicação
2024
Arquivos neste item
Autoaggregation_shaking.pdf
Descrição: Autoaggregation assays under shaking incubation at 37°C. All cultures were homogenized before each reading. Missing values represent outliers that were removed or strains that were not tested on a specific experiment. OD readings at 600 nm.
Tamanho: 174.79 kB
Formato: Adobe PDF
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Descrição: Autoaggregation assays under shaking incubation at 37°C. All cultures were homogenized before each reading. Missing values represent outliers that were removed or strains that were not tested on a specific experiment. OD readings at 600 nm.
Tamanho: 174.79 kB
Formato: Adobe PDF
Ver/Aberto
Autoaggregation assays_static conditions.pdf
Descrição: Autoaggregation assays performed under static conditions at 37°C. All cultures were homogenized at the start of experiment and then kept at static conditions for further measurements. The missing values represent an outlier that was removed or strains that were not used on specific experiments. OD readings at 600 nm.
Tamanho: 168.71 kB
Formato: Adobe PDF
Ver/Aberto
Descrição: Autoaggregation assays performed under static conditions at 37°C. All cultures were homogenized at the start of experiment and then kept at static conditions for further measurements. The missing values represent an outlier that was removed or strains that were not used on specific experiments. OD readings at 600 nm.
Tamanho: 168.71 kB
Formato: Adobe PDF
Ver/Aberto
Biofilm assays_polystyrene.pdf
Descrição: Biofilm assays performed on polystyrene surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 126.18 kB
Formato: Adobe PDF
Ver/Aberto
Descrição: Biofilm assays performed on polystyrene surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 126.18 kB
Formato: Adobe PDF
Ver/Aberto
Biofilm assays_glass.pdf
Descrição: Biofilm assays performed on glass surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 129.3 kB
Formato: Adobe PDF
Ver/Aberto
Descrição: Biofilm assays performed on glass surface after 24 h incubation under static condition at 37°C. Missing values represent outliers that were removed. OD readings at 570 nm of solubilized crystal violet in ethanol.
Tamanho: 129.3 kB
Formato: Adobe PDF
Ver/Aberto
ELISA_Binding to ECM components.pdf
Descrição: ELISA assays to detect binding to extracellular matrix components. Adherence to 1 µg of the extracellular matrix components assay performed using 0.1 µM of the passenger domain (recombinant protein) of Eaa. Readings were taken at 492 nm. Values represent the average of each experiment.
Tamanho: 160.08 kB
Formato: Adobe PDF
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Descrição: ELISA assays to detect binding to extracellular matrix components. Adherence to 1 µg of the extracellular matrix components assay performed using 0.1 µM of the passenger domain (recombinant protein) of Eaa. Readings were taken at 492 nm. Values represent the average of each experiment.
Tamanho: 160.08 kB
Formato: Adobe PDF
Ver/Aberto
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