PhcrTx2, a new crab-paralyzing peptide toxin from the sea anemone phymanthus crucifer
Universidade de Ulm¦¦Alemanha ; Hannover Medical School (MHH)¦¦Alemanha ; Benemérita Universidad Autónoma de Puebla (BUAP)¦¦México ; Universidade Católica de Leuven¦¦Bélgica ; Consejo Superior de Investigaciones Cientificas (CSIC)¦¦Espanha ; University of Florida (UF)¦¦Estados Unidos ; Universidad Nacional Autónoma de México (UNAM)¦¦México ; Centro de Bioproductos Marinos (CEBIMAR)¦¦Cuba
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Sea anemones produce proteinaceous toxins for predation and defense, including peptide toxins that act on a large variety of ion channels of pharmacological and biomedical interest. Phymanthus crucifer is commonly found in the Caribbean Sea; however, the chemical structure and biological activity of its toxins remain unknown, with the exception of PhcrTx1, an acid-sensing ion channel (ASIC) inhibitor. Therefore, in the present work, we focused on the isolation and characterization of new P. crucifer toxins by chromatographic fractionation, followed by a toxicity screening on crabs, an evaluation of ion channels, and sequence analysis. Five groups of toxic chromatographic fractions were found, and a new paralyzing toxin was purified and named PhcrTx2. The toxin inhibited glutamate-gated currents in snail neurons (maximum inhibition of 35%, IC50 4.7 mu M), and displayed little or no influence on voltage-sensitive sodium/potassium channels in snail and rat dorsal root ganglion (DRG) neurons, nor on a variety of cloned voltage-gated ion channels. The toxin sequence was fully elucidated by Edman degradation. PhcrTx2 is a new -defensin-fold peptide that shares a sequence similarity to type 3 potassium channels toxins. However, its low activity on the evaluated ion channels suggests that its molecular target remains unknown. PhcrTx2 is the first known paralyzing toxin in the family Phymanthidae.
Rodriguez AA, Garateix A, Salceda E, Peigneur S, Zaharenko AJ, Pons T, et al. PhcrTx2, a new crab-paralyzing peptide toxin from the sea anemone phymanthus crucifer. Toxins. 2018 Feb;10(2):72. doi:10.3390/toxins10020072.
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Fundação Alexander von Humboldt ; German Research Foundation (DFG) ; International Foundation for Science (IFS) ; Organização Europeia de Biologia Molecular (EMBO) ; Academia Mundial de Ciências (TWAS) Itália; Government of Veracruz (Mexico) ; Consejo Nacional de Ciencia y Tecnología (CONACYT) ; VIEP - Benemérita Universidad Autónoma de Puebla ; Programa de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIE) ; Programa Iberoamericano de Ciencia y Tecnología (CYTED) ; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; Fonds Wetenschappelijk Onderzoek - Vlaanderen ; Interuniversity Attraction Poles (IAP) Programme of the Belgian Science Policy Office ; Universidade Católica de Leuven
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