Sexual and ontogenetic variation of Bothrops leucurus venom


Publication type
Article
Language
English
Appears in Collections:
Metrics
Abstract
Various factors, such as geographical origin, climate, sex, age and diet can influence the composition and pathophysiological activities of snake venoms. In this study, we examined the sexual and ontogenetic variations in the venom of Bothrops leucurus, a pitviper responsible for more than 80% of the snakebites in the state of Bahia, northeastern Brazilian. The venoms of 31 snakes were pooled according to sex and age (young, adult and old) and screened by SDS-PAGE (in reducing and non-reducing conditions), reverse-phase high performance liquid chromatography (RP-HPLC), gelatin zymography, and immunoblotting with therapeutic bothropic antivenom (BAV) from the Instituto Butantan. The electrophoretic and chromatographic profiles showed intraspecific ontogenetic variation, whereas sexual variations were less evident. All venoms showed gelatinolytic activity associated with 50–75 kDa protein bands. In addition, all venoms, regardless of the snakes' sex and age, cross-reacted to similar extents with BAV. Our findings show that B. leucurus venom changes during ontogenetic development and demonstrate sexual differences in its composition, indicating differences in biological activity.
Reference
Braga JRM, Morais-Zani K, Pereira DS, Sant'Anna SS, Galizio NC, Tanaka-Azevedo AM, et al. Sexual and ontogenetic variation of Bothrops leucurus venom. Toxicon. 2020 Sep;184:127-135. doi:10.1016/j.toxicon.2020.05.028.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/3073
Journal title
Issue Date
2020


Files in This Item:

Existing users please Login
10.1016j.toxicon.2020.05.028.pdf
Size: 1.52 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show full item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.