Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria

The application of next-generation molecular, biochemical and immunological methods for developing new vaccines, antimicrobial compounds, probiotics and prebiotics for zoonotic infection control has been fundamental to the understanding and preservation of the symbiotic relationship between animals and humans. With increasing rates of antibiotic use, resistant bacterial infections have become more difficult to diagnose, treat, and eradicate, thereby elevating the importance of surveillance and prevention programs. Effective surveillance relies on the availability of rapid, cost-effective methods to monitor pathogenic bacterial isolates. In this opinion article, we summarize the results of some research program initiatives for the improvement of live vaccines against avian enterotoxigenic Escherichia coli using virulence factor gene deletion and engineered vaccine vectors based on probiotics. We also describe methods for the detection of pathogenic bacterial strains in eco-environmental headspace and aerosols, as well as samples of animal and human breath, based on the composition of volatile organic compounds and fatty acid methyl esters. We explain how the introduction of these low-cost biotechnologies and protocols will provide the opportunity to enhance co-operation between networks of resistance surveillance programs and integrated routine workflows of veterinary and clinical public health microbiology laboratories.
Keywords
Bacterial vaccines;  Microbiomes;  Probiotics;  Antibiotics;  VOCs;  And FAMEs

metadata.dc.contributor
metadata.dc.contributor.external
metadata.dc.description.sponsorship
Document type
Article
Source
Belizário JE., Sircili MP. Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria. BMC Vet. Res.. 2020 Nov;16:420. doi:10.1186/s12917-020-02633-8.
Appears in Collections:
Metrics
Rights
Open Access
URL
URI

Files in This Item:
File Description SizeFormat Existing users please Login
10.1186s12917-020-02633-8.pdf1.01 MBAdobe PDFEmbargoed until January 1, 2999    Request a copy
Show full item record

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.